圆葱（Allium rotundum L.）精油和甲醇提取物的体外抗氧化及抗溶血作用

In vitro antioxidant and antihemolytic effects of the essential oil and methanolic extract of Allium rotundum L.

作者信息

Assadpour S, Nabavi S M, Nabavi S F, Dehpour A A, Ebrahimzadeh M A

机构信息

Pharmaceutical Sciences Research Center, School of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran.

出版信息

Eur Rev Med Pharmacol Sci. 2016 Dec;20(24):5210-5215.

PMID:28051246

Abstract

OBJECTIVE

A plethora of scientific evidence showed that several plant species from the genus Allium (Alliaceae) possess multiple therapeutic effects. Present paper aimed to examine the antioxidant and antihemolytic activities of the essential oil and methanol extract Allium rotundum L. through different in vitro assays.

MATERIALS AND METHODS

1,1-diphenyl-2-picryl hydroxyl radical (DPPH), nitric oxide as well as hydrogen peroxide scavenging, Fe2+ chelating, reducing power and also hemoglobin-induced linoleic acid peroxidation assay systems have been utilized to examine antioxidant effects of these samples. Total amounts of phenolic and flavonoid contents were calculated. The antihemolytic effect was investigated against hemolysis induced by hydrogen peroxide in rat erythrocytes. Also, mineral contents of plant were evaluated by atomic absorption spectroscopy.

RESULTS

IC50 for DPPH radical-scavenging activity were 284 ± 11.64 for methanol extract and 1264 ± 45.60 µg ml-1 for essential oil, respectively. The extract has shown better reducing effects versus essential oil. The extract also demonstrated better activity in nitric oxide-scavenging activity. IC50 were 464 ± 19.68 for extract and 1093 ± 38.25 µg ml-1 for essential oil. The extract shows better activity than essential oil in Fe2+ chelating system. IC50 were 100 ± 3.75 for extract and 1223 ± 36.25 µg ml-1 for essential oil. The A. rotundum extract and essential oil showed significant H2O2 scavenging effects at dose-dependent manners. IC50 was 786 ± 29.08 mg ml-1 for essential oil. The amounts of eight elements were determined. The concentrations of elements were in the order: Mn> Fe> Zn> Cu> Ni> Cd.

CONCLUSIONS

The extract showed a higher antioxidant effect in all tested models including DPPH, nitric oxide, reducing power as well as iron chelating and antihemolytic activities than essential oil. The latter showed more potent antioxidant activity in scavenging H2O2 and lipid peroxidation model. Antioxidant activities of extract may be attributed at least in part, due to its phenolic and flavonoid contents.

摘要

目的

大量科学证据表明，葱属（葱科）的几种植物具有多种治疗作用。本文旨在通过不同的体外试验来检测圆葱精油和甲醇提取物的抗氧化和抗溶血活性。

材料与方法

利用1,1-二苯基-2-苦基肼自由基（DPPH）、一氧化氮以及过氧化氢清除、亚铁离子螯合、还原能力以及血红蛋白诱导的亚油酸过氧化试验系统来检测这些样品的抗氧化作用。计算酚类和黄酮类化合物的总量。研究了其对大鼠红细胞中过氧化氢诱导的溶血的抗溶血作用。此外，通过原子吸收光谱法评估了植物的矿物质含量。

结果

甲醇提取物和精油对DPPH自由基清除活性的IC50分别为284±11.64和1264±45.60μg/ml。提取物显示出比精油更好的还原效果。提取物在一氧化氮清除活性方面也表现出更好的活性。提取物和精油的IC50分别为464±19.68和1093±38.25μg/ml。提取物在亚铁离子螯合系统中比精油表现出更好的活性。提取物和精油的IC50分别为100±3.75和1223±36.25μg/ml。圆葱提取物和精油以剂量依赖的方式表现出显著的过氧化氢清除作用。精油的IC50为786±29.08mg/ml。测定了八种元素的含量。元素浓度顺序为：锰>铁>锌>铜>镍>镉。