Schleicher Martina, Wendel Hans Peter, Fritze Olaf, Stock Ulrich A
Department of Thoracic, Cardiac & Vascular Surgery, University Hospital Tuebingen, Hoppe-Seyler-Strasse 3, 72076 Tuebingen, Germany.
Regen Med. 2009 Jul;4(4):613-9. doi: 10.2217/rme.09.22.
Current tissue-engineering principles of heart valves include tissue- or stem cell-derived cells with subsequent in vitro incubation on various scaffolds prior to implantation. Limitations of this approach include a long in vitro culture, an accompanied risk of infection and sophisticated, cost-intensive infrastructures. An 'off-the-shelf' heart valve with in vivo endothelialization and tissue-regeneration potential would overcome these limitations. Additionally, the development of a heart valve with growth potential would be a huge improvement for pediatric patients. This article discusses different starter matrices, homing and immobilization strategies of host cells and masking approaches of inflammatory structures for in vivo surface and tissue engineering of heart valves. Novel concepts will be presented based on highly specific DNA-aptamers immobilized on the heart valve surface as capture molecules for endothelial progenitor cells circulating in the bloodstream.
当前心脏瓣膜组织工程原理包括使用组织或干细胞来源的细胞，在植入前先在各种支架上进行体外培养。这种方法的局限性包括体外培养时间长、伴随感染风险以及复杂且成本高昂的基础设施。具有体内内皮化和组织再生潜力的“现成可用”心脏瓣膜将克服这些局限性。此外，开发具有生长潜力的心脏瓣膜对儿科患者来说将是一个巨大的进步。本文讨论了用于心脏瓣膜体内表面和组织工程的不同起始基质、宿主细胞的归巢和固定策略以及炎症结构的屏蔽方法。将基于固定在心脏瓣膜表面的高度特异性DNA适体作为捕获血液中循环的内皮祖细胞的分子，提出新的概念。
