Yoon Angela J, Shen Jing, Wu Hui-Chen, Angelopoulos Christos, Singer Steven R, Chen Rongzhen, Santella Regina M
Columbia University College of Dental Medicine, Columbia University Medical Center, 630 West 168th Street, PH15W-1562, New York, NY 10032, USA.
Radiat Res. 2009 Jun;171(6):771-5. doi: 10.1667/RR1560.1.
Gamma-H2AX (activated histone 2AX) and pChk2 (activated checkpoint kinase 2), which are DNA damage response molecules, are produced in irradiated cells and may be signature molecules of radiation exposure. We investigated their use as potential biomarkers to identify individuals exposed to ionizing radiation. We collected exfoliated oral epithelial cell samples from 100 healthy individuals undergoing routine dental radiographic examination (2.34 cGy) both before and after the radiograph using a non-invasive technique. The expression levels of pChk2 and gamma-H2AX in oral cells were assessed by immunohistochemical assay. Both biomarkers showed statistically significant increases in levels of expression after the radiation exposure (P < 0.001). This suggests that pChk2 and gamma-H2AX may serve as sensitive indicators of low-dose radiation exposure.
γ-H2AX（活化组蛋白2AX）和pChk2（活化的检查点激酶2）是DNA损伤反应分子，在受辐照细胞中产生，可能是辐射暴露的标志性分子。我们研究了它们作为潜在生物标志物用于识别暴露于电离辐射的个体的情况。我们使用非侵入性技术，在进行常规牙科X线检查（2.34 cGy）的100名健康个体进行X线检查之前和之后，收集了脱落的口腔上皮细胞样本。通过免疫组织化学分析评估口腔细胞中pChk2和γ-H2AX的表达水平。两种生物标志物在辐射暴露后表达水平均有统计学显著增加（P < 0.001）。这表明pChk2和γ-H2AX可能作为低剂量辐射暴露的敏感指标。
