Morphology Group, Biomedical Research Institute, Hasselt University, Agoralaan Building C, Diepenbeek, Belgium.
Belgian Nuclear Research Centre, Radiobiology Unit, SCK•CEN, Mol, Belgium.
Sci Rep. 2020 Feb 7;10(1):2113. doi: 10.1038/s41598-020-58746-5.
Assessing the possible biological effects of exposure to low doses of ionizing radiation (IR) is one of the prime challenges in radiation protection, especially in medical imaging. Today, radiobiological data on cone beam CT (CBCT) related biological effects are scarce. In children and adults, the induction of DNA double strand breaks (DSBs) in buccal mucosa cells and 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxo-dG) and antioxidant capacity in saliva samples after CBCT examination were examined. No DNA DSBs induction was observed in children nor adults. In children only, an increase in 8-oxo-dG levels was observed 30 minutes after CBCT. At the same time an increase in antioxidant capacity was observed in children, whereas a decrease was observed in adults. Our data indicate that children and adults react differently to IR doses associated with CBCT. Fully understanding these differences could lead to an optimal use of CBCT in different age categories as well as improved radiation protection guidelines.
评估低剂量电离辐射（IR）暴露的可能生物学效应是辐射防护的主要挑战之一，尤其是在医学成像领域。目前，关于锥形束 CT（CBCT）相关生物学效应的放射生物学数据还很有限。本研究检测了儿童和成人在接受 CBCT 检查后颊黏膜细胞中 DNA 双链断裂（DSBs）的诱导情况以及唾液样本中的 8-氧代-7,8-二氢-2'-脱氧鸟苷（8-oxo-dG）和抗氧化能力。结果未发现儿童或成人的 DNA DSBs 诱导。仅在儿童中，在 CBCT 检查后 30 分钟观察到 8-oxo-dG 水平升高。与此同时，在儿童中观察到抗氧化能力增加，而在成人中则观察到抗氧化能力下降。我们的数据表明，儿童和成人对与 CBCT 相关的 IR 剂量的反应不同。充分了解这些差异可能会导致在不同年龄段更优地使用 CBCT，以及改进辐射防护指南。
