口腔黏膜细胞的细胞遗传学生物监测在牙科 X 射线之后。
Cytogenetic biomonitoring in oral mucosa cells following dental X-ray.
机构信息
Department of Biosciences, Federal University of Sao Paulo, Brazil.
出版信息
Dentomaxillofac Radiol. 2012 Mar;41(3):181-4. doi: 10.1259/dmfr/14555883.
OBJECTIVES
In the past decades, X-rays have been used widely for diagnosis in dentistry. However, it is well known that ionizing radiation causes damage (including single- and double-strand breaks) to deoxyribonucleic acid (DNA) and DNA-protein crosslinks, and induces cellular death. Therefore, outlining the cytogenetic effects induced by X-ray is necessary to identify the degree of cancer risk and minimize potential risks to patients and clinicians. To date, a variety of assays have been proposed in cytogenetic biomonitoring studies, including those that assess metaphase chromosomal aberrations and sister chromatid exchanges, and micronucleus and single-cell gel (comet) assay.
METHODS
Cytogenetic biomonitoring studies focusing on oral mucosa cells in individuals exposed to dental X-ray were reviewed.
RESULTS
Dental X-ray can induce DNA damage and cytotoxicity in oral mucosa cells.
CONCLUSION
These results will contribute to a better understanding of X-ray-induced effects upon the cellular system in individuals continually exposed to known genotoxic/cytotoxic agents.
目的
在过去几十年中，X 射线已广泛应用于牙科诊断。然而，众所周知，电离辐射会对脱氧核糖核酸（DNA）和 DNA-蛋白质交联造成损伤（包括单链和双链断裂），并诱导细胞死亡。因此，需要阐明 X 射线引起的细胞遗传学效应，以确定癌症风险程度，并将患者和临床医生的潜在风险降至最低。迄今为止，已经在细胞遗传学生物监测研究中提出了多种检测方法，包括评估中期染色体畸变和姐妹染色单体交换，以及微核和单细胞凝胶（彗星）试验。
方法
综述了针对暴露于牙科 X 射线的个体口腔黏膜细胞的细胞遗传学生物监测研究。
结果
牙科 X 射线可诱导口腔黏膜细胞中的 DNA 损伤和细胞毒性。
结论
这些结果将有助于更好地了解持续暴露于已知遗传毒性/细胞毒性剂的个体中，X 射线对细胞系统的诱导作用。
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