Guo Xueqing, Wang Qianli, Swarts Benjamin M, Guo Zhongwu
Department of Chemistry, Wayne State University, 5101 Cass Avenue, Detroit, Michigan 48202, USA.
J Am Chem Soc. 2009 Jul 29;131(29):9878-9. doi: 10.1021/ja903231v.
It is demonstrated that sortase A (SrtA) can catalyze efficient coupling of peptides to GPI analogues with a glycine residue attached to the phosphoethanolamine moiety at the nonreducing end to form GPI-linked peptides. This represents the first chemoenzymatic synthesis of GPI-peptide conjugates and is a proof-of-concept for the potential application of SrtA to the synthesis of more complex GPI-anchored peptides/glycopeptides and GPI-anchored proteins/glycoproteins.
已证明分选酶A（SrtA）可以催化肽与GPI类似物的有效偶联，该GPI类似物在非还原端的磷酸乙醇胺部分连接有甘氨酸残基，以形成GPI连接的肽。这代表了GPI-肽缀合物的首次化学酶促合成，并且是SrtA在合成更复杂的GPI锚定肽/糖肽和GPI锚定蛋白/糖蛋白方面潜在应用的概念验证。
