Gronemeyer H, Meyer M E, Bocquel M T, Kastner P, Turcotte B, Chambon P
Laboratoire de Génétique Moléculaire des Eucaryotes du CNRS, Institut de Chimie Biologique, Faculté de Médecine, Strasbourg, France.
J Steroid Biochem Mol Biol. 1991;40(1-3):271-8. doi: 10.1016/0960-0760(91)90192-8.
We present evidence that the two isoforms of A and B of the chicken (cPR) and human progesterone receptor (hPR) originate from two different mRNA populations. One of these encodes the isoforms A which originate by initiation of translation at an in-frame AUG found 127 (cPR) and 165 (hPR) codons downstream of the AUG which gives rise to the isoforms B. Two estrogen-inducible hPR promoters were identified which are responsible for the generation of these two classes of transcripts. Characterization of the cPR promoter suggested the possible existence of cell-type and isoform-specific auto-regulation of cPR transcription and provided evidence that estrogen-induction of cPR expression occurs at a post-transcriptional level. Finally, we demonstrate promoter-specific transcriptional activation by the hPR isoforms A and B, and we discuss the mechanism of action of the anti-progestin RU486.
我们提供的证据表明，鸡孕酮受体（cPR）和人孕酮受体（hPR）的A和B两种亚型源自两个不同的mRNA群体。其中一个群体编码A亚型，它是通过在位于产生B亚型的AUG下游127个（cPR）和165个（hPR）密码子处的框内AUG起始翻译而产生的。鉴定出了两个雌激素诱导型hPR启动子，它们负责产生这两类转录本。cPR启动子的特性表明可能存在cPR转录的细胞类型和亚型特异性自动调节，并提供了证据表明cPR表达的雌激素诱导发生在转录后水平。最后，我们证明了hPR A和B亚型的启动子特异性转录激活，并讨论了抗孕激素RU486的作用机制。
