Department of Genetics, Faculty of Natural Sciences, Comenius University, Mlynska dolina B1, 842 15 Bratislava, Slovakia.
Phytother Res. 2010 Jan;24(1):90-5. doi: 10.1002/ptr.2901.
The potential genotoxicity and antigenotoxicity of non-photoactivated hypericin was investigated in five experimental models. Hypericin was non-mutagenic in the Ames assay, with and without metabolic activation. It did not exert a protective effect against mutagenicity induced by 9-aminoacridine. In a yeast (Saccharomyces cerevisiae) assay, hypericin did not increase the frequency of mitotic crossovers or total aberrants at the ade(2) locus, the number of convertants at the trp5 locus, or the number of revertants at the ilv1 locus. In combined application with 4-nitroquinoline-1-oxide, it significantly enhanced the number of revertants at the ilv1 locus at the highest concentration used. Hypericin was not mutagenic in the alga Chlamydomonas reinhardtii. However, in combined application with methyl methane sulfonate, toxicity and mutagenicity were slightly reduced. In a chromosome aberration assay using three mammalian cell lines, hypericin did not alter the frequency of structural chromosome aberrations, and in the DPPH radical scavenging assay, it did not exert any antioxidant effects.
本研究旨在探讨非光活化金丝桃素的潜在遗传毒性和抗原毒性。在 5 种实验模型中，金丝桃素在 Ames 试验中无论是否存在代谢激活均无致突变性。它对吖啶引起的致突变性没有保护作用。在酵母（酿酒酵母）试验中，金丝桃素不会增加 ade(2)基因座的有丝分裂交叉或总异常的频率，也不会增加 trp5 基因座的转换体数量或 ilv1 基因座的回复体数量。在与 4-硝基喹啉-1-氧化物联合应用时，在使用的最高浓度下，金丝桃素显著增加了 ilv1 基因座的回复体数量。金丝桃素在绿藻衣藻中没有致突变性。然而，在与甲磺酸甲酯联合应用时，毒性和致突变性略有降低。在使用三种哺乳动物细胞系的染色体畸变试验中，金丝桃素不会改变结构染色体畸变的频率，并且在 DPPH 自由基清除试验中，它没有表现出任何抗氧化作用。
