Tavares Denise Crispim, Mazzaron Barcelos Gustavo Rafael, Silva Lívia Ferreira, Chacon Tonin Conception Cortez, Bastos Jairo Kenupp
Universidade de Franca, Av. Armando Salles de Oliveira, 201, 14404-600 Franca, São Paulo, Brazil.
Toxicol In Vitro. 2006 Oct;20(7):1154-8. doi: 10.1016/j.tiv.2006.02.009. Epub 2006 Mar 3.
Propolis has been used in folk medicine since ancient times and is known for its antimicrobial, antiparasitic, antiviral, anti-inflammatory, antitumoral and antioxidant properties. In view of the great therapeutic interest in propolis and the small number of studies regarding its mechanism of action, the aim of the present study was to evaluate the mutagenic and antimutagenic effects of propolis using Chinese hamster ovary cells. Parameters such as the frequency of chromosome aberrations and mitotic index were analyzed. The results showed that, on one hand, the highest propolis tested concentration displayed a small but significant increase in the frequency of chromosome aberrations, and on the other hand, it was observed that the lowest tested concentration significantly reduced the chromosome damage induced by the chemotherapeutic agent doxorubicin. The present results indicate that propolis shows the characteristic of a "Janus" compound, i.e., propolis is genotoxic at higher concentrations, while at lower concentrations it display a chemopreventive effect on doxorubicin-induced mutagenicity. Flavonoids may be the components of propolis responsible for its both mutagenic and antimutagenic effects, once these compounds may act either as pro-oxidant or as free radicals scavenger, depending on its concentration.
蜂胶自古以来就在民间医学中使用，因其具有抗菌、抗寄生虫、抗病毒、抗炎、抗肿瘤和抗氧化特性而闻名。鉴于对蜂胶的巨大治疗兴趣以及关于其作用机制的研究数量较少，本研究的目的是使用中国仓鼠卵巢细胞评估蜂胶的致突变和抗突变作用。分析了诸如染色体畸变频率和有丝分裂指数等参数。结果表明，一方面，所测试的最高蜂胶浓度显示染色体畸变频率有小幅但显著的增加，另一方面，观察到所测试的最低浓度显著降低了化疗药物阿霉素诱导的染色体损伤。目前的结果表明，蜂胶表现出“两面神”化合物的特征，即蜂胶在较高浓度下具有遗传毒性，而在较低浓度下对阿霉素诱导的致突变性具有化学预防作用。黄酮类化合物可能是蜂胶产生致突变和抗突变作用的成分，因为这些化合物根据其浓度可作为促氧化剂或自由基清除剂起作用。
