Okazaki Kazunobu, Hayase Hideki, Ichinose Takashi, Mitono Hiroyuki, Doi Tatsuya, Nose Hiroshi
Department of Sports Medical Sciences, Shinshu University Graduate School of Medicine, Matsumoto 390-8621, Japan.
J Appl Physiol (1985). 2009 Sep;107(3):770-9. doi: 10.1152/japplphysiol.91264.2008. Epub 2009 Jul 9.
This study examined whether increased plasma volume (PV) and albumin content (Alb(cont)) in plasma for 23 h after exercise were attenuated in older subjects compared with in young adult subjects, and if this attenuation abated by supplementation with protein and carbohydrate (CHO) immediately after exercise. Eight moderately active older (approximately 68 yr) and 8 young (approximately 21 yr) men performed two trials: control (CNT) and Pro-CHO in which subjects consumed placebo (0.5 kcal, 0 g protein, 0.5 mg Na(+) in 3.2 ml total fluid volume/kg body wt) or protein and CHO mixture (3.2 kcal, 0.18 g protein, 0.5 mg Na(+) in 3.2 ml total fluid volume/kg body wt) supplementations, respectively, immediately after high-intensity interval exercise for 72 min [8 sets of 4 min at 70-80% peak oxygen consumption rate (Vo(2peak)) intermitted by 5 min at 20% Vo(2peak)]. PV, Alb(cont), and plasma globulin content (Glb(cont)) were measured before exercise, at the end of exercise, every hour from the 1st to the 5th hour after exercise, and at the 23rd hour after exercise. From 12 h before the start to the end of experiment, food intake was controlled to the age-matched recommended dietary allowances. We found that during the first 4 h after exercise in CNT, Alb(cont) recovered less in the older than the young group by approximately 0.04 g/kg (P < 0.05), while it generally recovered more with Pro-CHO than CNT by approximately 0.09 and approximately 0.04 g/kg in the young and older group, respectively, accompanied by a greater increase in PV by approximately 1 and approximately 2 ml/kg, respectively, during the 23 h after exercise (P < 0.05). Glb(cont) remained constant throughout the experiment in both trials for both age groups. Thus the attenuated responses of Alb(cont) and PV after exercise in older subjects were restored by protein and CHO supplementation immediately after exercise, similarly to young subjects.
本研究调查了与年轻成年受试者相比，老年受试者运动后23小时血浆容量（PV）增加及血浆白蛋白含量（Alb(cont)）是否减弱，以及运动后立即补充蛋白质和碳水化合物（CHO）是否能减轻这种减弱。8名中度活跃的老年男性（约68岁）和8名年轻男性（约21岁）进行了两项试验：对照试验（CNT）和Pro-CHO试验，受试者分别在高强度间歇运动72分钟后立即摄入安慰剂（0.5千卡，0克蛋白质，每千克体重3.2毫升总液体量中含0.5毫克钠）或蛋白质和CHO混合物（3.2千卡，0.18克蛋白质，每千克体重3.2毫升总液体量中含0.5毫克钠）。在运动前、运动结束时、运动后第1至5小时每小时以及运动后第23小时测量PV、Alb(cont)和血浆球蛋白含量（Glb(cont)）。从实验开始前12小时至实验结束，食物摄入量控制在与年龄匹配的推荐膳食摄入量水平。我们发现，在CNT试验中，运动后前4小时，老年组Alb(cont)的恢复比年轻组少约0.04克/千克（P<0.05），而在Pro-CHO试验中，年轻组和老年组Alb(cont)的恢复通常比CNT试验分别多约0.09和0.04克/千克，运动后23小时PV分别增加约1和2毫升/千克（P<0.05）。在两个年龄组的两项试验中，Glb(cont)在整个实验过程中均保持恒定。因此，与年轻受试者类似，运动后立即补充蛋白质和CHO可恢复老年受试者运动后Alb(cont)和PV减弱的反应。
