Department of Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, TX 77030, USA.
J Fluoresc. 2009 Nov;19(6):1103-9. doi: 10.1007/s10895-009-0510-y. Epub 2009 Jul 10.
mCherry is a red fluorescent protein which is bright, photostable, and has a low molecular weight. It is an attractive choice for multiphoton fluorescence imaging; however, the multiphoton excitation spectrum of mCherry is not known. In this paper we report the two photon excitation spectrum of mCherry measured up to 1190 nm in the near infrared (NIR) region. Skin tissues of transgenic mice that express mCherry were used in the experiments. mCherry in the tissues was excited with a Titanium:Sapphire laser and an optical parametric oscillator pumped by the Titanium:Sapphire laser. We found that the peak excitation of mCherry occurs at 1160 nm.
mCherry 是一种红色荧光蛋白，具有亮度高、光稳定性好和分子量低的特点。它是多光子荧光成像的理想选择；然而，mCherry 的多光子激发光谱尚不清楚。本文报道了在近红外（NIR）区域高达 1190nm 处测量的 mCherry 的双光子激发光谱。实验中使用了表达 mCherry 的转基因小鼠的皮肤组织。用钛宝石激光器激发组织中的 mCherry，并利用钛宝石激光器泵浦的光学参量振荡器进行激发。我们发现 mCherry 的峰值激发发生在 1160nm。
