使用光学参量振荡器（OPO）对 mCherry 进行峰值多光子激发。
Peak multiphoton excitation of mCherry using an optical parametric oscillator (OPO).
机构信息
Department of Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, TX 77030, USA.
出版信息
J Fluoresc. 2009 Nov;19(6):1103-9. doi: 10.1007/s10895-009-0510-y. Epub 2009 Jul 10.
Abstract
mCherry is a red fluorescent protein which is bright, photostable, and has a low molecular weight. It is an attractive choice for multiphoton fluorescence imaging; however, the multiphoton excitation spectrum of mCherry is not known. In this paper we report the two photon excitation spectrum of mCherry measured up to 1190 nm in the near infrared (NIR) region. Skin tissues of transgenic mice that express mCherry were used in the experiments. mCherry in the tissues was excited with a Titanium:Sapphire laser and an optical parametric oscillator pumped by the Titanium:Sapphire laser. We found that the peak excitation of mCherry occurs at 1160 nm.
摘要
mCherry 是一种红色荧光蛋白，具有亮度高、光稳定性好和分子量低的特点。它是多光子荧光成像的理想选择；然而，mCherry 的多光子激发光谱尚不清楚。本文报道了在近红外（NIR）区域高达 1190nm 处测量的 mCherry 的双光子激发光谱。实验中使用了表达 mCherry 的转基因小鼠的皮肤组织。用钛宝石激光器激发组织中的 mCherry，并利用钛宝石激光器泵浦的光学参量振荡器进行激发。我们发现 mCherry 的峰值激发发生在 1160nm。
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Curr Opin Biotechnol. 2009 Feb;20(1):106-10. doi: 10.1016/j.copbio.2009.02.009. Epub 2009 Mar 9.
2
A membrane associated mCherry fluorescent reporter line for studying vascular remodeling and cardiac function during murine embryonic development.一种用于研究小鼠胚胎发育过程中血管重塑和心脏功能的膜相关mCherry荧光报告基因系。
Anat Rec (Hoboken). 2009 Mar;292(3):333-41. doi: 10.1002/ar.20821.
3
Bright far-red fluorescent protein for whole-body imaging.用于全身成像的明亮远红荧光蛋白。
Nat Methods. 2007 Sep;4(9):741-6. doi: 10.1038/nmeth1083. Epub 2007 Aug 26.
4
Principles of two-photon excitation fluorescence microscopy and other nonlinear imaging approaches.双光子激发荧光显微镜及其他非线性成像方法的原理。
Adv Drug Deliv Rev. 2006 Sep 15;58(7):788-808. doi: 10.1016/j.addr.2006.07.005. Epub 2006 Aug 9.
5
A guide to choosing fluorescent proteins.荧光蛋白选择指南。
Nat Methods. 2005 Dec;2(12):905-9. doi: 10.1038/nmeth819.
6
Using a histone yellow fluorescent protein fusion for tagging and tracking endothelial cells in ES cells and mice.利用组蛋白黄色荧光蛋白融合技术标记和追踪胚胎干细胞及小鼠中的内皮细胞。
Genesis. 2005 Jul;42(3):162-71. doi: 10.1002/gene.20139.
7
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