Mohamed Mahmoud H A, Kumar Sachin, Paldurai Anandan, Megahed Mohamed M, Ghanem Ibrahim A, Lebdah Mohamed A, Samal Siba K
Virginia-Maryland Regional College of Veterinary Medicine, University of Maryland, College Park, MD 20742, USA.
Virus Genes. 2009 Oct;39(2):234-7. doi: 10.1007/s11262-009-0385-7.
The complete genome sequence of a virulent Newcastle disease virus (NDV) isolated from chickens in Egypt was determined and compared to the sequence of NDV strains isolated from different parts of the world. The genome is 15,186 nucleotides (nt) long and consists of 6 genes in the order of 3'-N-P-M-F-HN-L-5'. The genome contains a 55-nt leader region at the 3' end and a 114-nt trailer region at the 5' end. Interestingly, the phylogenetic analysis showed that strain Egypt is closely related with the NDV strains isolated in China. In addition, the sequence of the fusion protein cleavage site of strain Egypt was identical to that of the NDV strain recently isolated in Mali. Determination of complete genome sequences of additional NDV strains from Africa is necessary to understand the epidemiology of currently circulating viruses in Africa.
测定了从埃及鸡群中分离出的一种强毒新城疫病毒（NDV）的全基因组序列，并与从世界不同地区分离出的NDV毒株序列进行了比较。该基因组长度为15186个核苷酸（nt），由6个基因组成，顺序为3'-N-P-M-F-HN-L-5'。基因组在3'端含有一个55 nt的前导区，在5'端含有一个114 nt的尾随区。有趣的是，系统发育分析表明埃及毒株与在中国分离出的NDV毒株密切相关。此外，埃及毒株融合蛋白裂解位点的序列与最近在马里分离出的NDV毒株相同。有必要测定来自非洲的其他NDV毒株的全基因组序列，以了解非洲目前流行病毒的流行病学情况。
