Krishnamurthy S, Samal S K
Virginia-Maryland Regional College of Veterinary Medicine, University of Maryland, College Park 20742, USA.
J Gen Virol. 1998 Oct;79 ( Pt 10):2419-24. doi: 10.1099/0022-1317-79-10-2419.
The nucleotide sequences of the nucleocapsid protein (NP) gene, the intergenic regions in the nucleocapsid protein (NP)-phosphoprotein (P), P-matrix protein (M) and M-fusion glycoprotein gene junctions and the trailer region of a virulent Newcastle disease virus (NDV) strain Beaudette C were determined. The NP gene is 1747 nt long and encodes a protein of 489 amino acids. Each of the intergenic sequences determined is 1 nt long and, including the previously published intergenic sequences, the gene junction sequences varied in length from 1-47 nt and lacked any sequence identity. The 5' trailer region is 113 nt in length. Comparison of the sequences of the terminal leader and trailer regions of Beaudette C strain with those of nonvirulent strain B1 showed a high level of conservation, indicating the likelihood of these elements not being a factor in virulence. Together with previously published data, this report completes the sequence of the 15,186 nt genomic RNA of NDV strain Beaudette C.
测定了一株强毒新城疫病毒（NDV）Beaudette C株核衣壳蛋白（NP）基因、核衣壳蛋白（NP）-磷蛋白（P）、P-基质蛋白（M）和M-融合糖蛋白基因连接处的基因间隔区以及拖尾区的核苷酸序列。NP基因长1747个核苷酸，编码一个由489个氨基酸组成的蛋白质。所测定的每个基因间隔序列长1个核苷酸，并且包括先前发表的基因间隔序列在内，基因连接序列长度从1至47个核苷酸不等，且不存在任何序列同一性。5′拖尾区长113个核苷酸。将Beaudette C株的末端前导区和拖尾区序列与无毒力的B1株的相应序列进行比较，结果显示高度保守，表明这些元件不太可能是毒力的决定因素。结合先前发表的数据，本报告完成了NDV Beaudette C株15,186个核苷酸的基因组RNA序列测定。
