Liu Hualei, Chen Fei, Zhao Yunling, Zheng Dongxia, Li Jinming, Xu Tiangang, Qi Lin, Wang Zhiliang
National Reference Laboratory for Newcastle Disease, China Animal Health and Epidemiology Center, Qingdao 266032, China.
Virus Genes. 2010 Jun;40(3):365-71. doi: 10.1007/s11262-010-0452-0. Epub 2010 Feb 10.
The complete genomic sequence of Newcastle disease virus (NDV) strain NDV08-004, isolated from domestic ducks in China, was determined in this study. The genome is 15198 nucleotides (nt) in length, follows the "rule of six" and contains a 55-nt leader sequence at the 3' end and a 114-nt trailer sequence at the 5' end. Compared with the full genome sequences of Class II NDV strains, the NDV08-004 isolate has a 12-nt insertion (TGGGAGACGGGG) in the phosphoprotein gene between nucleotides 2381 and 2382 of the genome (numbered according to the genomic sequence of the La Sota strain, which consists of 15186 nt). Strain NDV08-004 has the motif (112)E-Q-Q-E-R-L(117) at the cleavage site of the fusion protein, which is typical of lentogenic NDV strains, and this is in agreement with the results of pathogenic tests based on the mean death time (MDT) and the intracerebral pathogenicity index (ICPI). Phylogenetic analysis based on the full genome revealed that all the NDV strains studied could be divided into two distinct clades, namely class I and class II, and the NDV08-004 isolate characterized in this study was grouped in class I. Further phylogenetic analysis based on a 374-bp fragment of the F gene in class I strains of NDV demonstrated that NDV08-004 belongs to genotype 3, and should be therefore similar to strains obtained from live bird markets in Hong Kong in recent years.
本研究测定了从中国家鸭中分离出的新城疫病毒（NDV）毒株NDV08 - 004的完整基因组序列。该基因组长度为15198个核苷酸（nt），遵循“六碱基规则”，在3'端含有一个55 nt的前导序列，在5'端含有一个114 nt的尾随序列。与II类NDV毒株的全基因组序列相比，NDV08 - 004分离株在基因组的2381和2382核苷酸之间的磷蛋白基因中有一个12 nt的插入片段（TGGGAGACGGGG）（根据由15186 nt组成的La Sota毒株的基因组序列编号）。NDV08 - 004毒株在融合蛋白的切割位点具有基序（112）E - Q - Q - E - R - L（117），这是弱毒株NDV的典型特征，这与基于平均死亡时间（MDT）和脑内致病性指数（ICPI）的致病性试验结果一致。基于全基因组的系统发育分析表明，所有研究的NDV毒株可分为两个不同的进化枝，即I类和II类，本研究中鉴定的NDV08 - 004分离株归为I类。基于NDV I类毒株F基因的374 bp片段的进一步系统发育分析表明，NDV08 - 004属于基因型3，因此应与近年来从香港活禽市场获得的毒株相似。
