Fowler K J, Clouston W M, Fournier R E, Evans B A
Howard Florey Institute of Experimental Physiology and Medicine, University of Melbourne, Parkville, Victoria, Australia.
FEBS Lett. 1991 Nov 4;292(1-2):183-6. doi: 10.1016/0014-5793(91)80863-x.
Relaxin is a polypeptide hormone that exerts a variety of physiological effects during pregnancy. To investigate the possibility that known genetic mutations affecting aspects of reproductive physiology result in alterations in the structure or production of relaxin, we have determined the chromosomal location of the mouse gene. The finding of a BamHI restriction fragment length polymorphism in AKR mice enabled us to use recombinant inbred strains to make an assignment to chromosome 19. This was confirmed by Southern analysis of DNA from microcell hybrids.
松弛素是一种多肽激素，在怀孕期间发挥多种生理作用。为了研究影响生殖生理各方面的已知基因突变是否会导致松弛素结构或产生的改变，我们确定了小鼠基因的染色体定位。在AKR小鼠中发现的BamHI限制性片段长度多态性使我们能够利用重组近交系将其定位到19号染色体上。通过对微细胞杂种DNA的Southern分析证实了这一点。
