Crawford R J, Hudson P, Shine J, Niall H D, Eddy R L, Shows T B
EMBO J. 1984 Oct;3(10):2341-5. doi: 10.1002/j.1460-2075.1984.tb02136.x.
We have recently cloned two different human relaxin gene sequences. One of these (H1) was isolated from a human genomic clone bank and the other (H2) from a cDNA library prepared from human pregnant ovarian tissue. Southern gel analysis of the relaxin genes within the genomes of several unrelated individuals showed that all genomes contained both relaxin genes. Hence it is unlikely (p less than 0.001) that the two relaxin gene sequences are alleles. Rather, it is probable that there are two relaxin genes within the human genome. It is likely that relaxin and insulin genes have evolved from a common ancestral gene by gene duplication, since structural similarities between insulin and relaxin are evident at both the peptide and gene level. To investigate the evolutionary relationship between the two human relaxin genes and the insulin gene, we have determined the chromosomal position of the relaxin genes using mouse/human cell hybrids. We found that the human insulin and relaxin genes are on different chromosomes. Both human relaxin genes are located on the short arm region of chromosome 9.
我们最近克隆了两种不同的人松弛素基因序列。其中一种（H1）是从人基因组克隆文库中分离出来的，另一种（H2）是从用人妊娠卵巢组织制备的cDNA文库中分离出来的。对几个不相关个体基因组中的松弛素基因进行的Southern凝胶分析表明，所有基因组都包含这两种松弛素基因。因此，这两种松弛素基因序列不太可能（p小于0.001）是等位基因。相反，人类基因组中可能存在两个松弛素基因。松弛素基因和胰岛素基因很可能是通过基因复制从一个共同的祖先基因进化而来的，因为胰岛素和松弛素在肽和基因水平上都存在明显的结构相似性。为了研究两个人松弛素基因与胰岛素基因之间的进化关系，我们利用小鼠/人细胞杂交体确定了松弛素基因的染色体位置。我们发现人胰岛素基因和松弛素基因位于不同的染色体上。两个人松弛素基因都位于9号染色体的短臂区域。
