小鼠中多肽激素基因的定位：生长抑素、胰高血糖素、降钙素和甲状旁腺激素。

Mapping polypeptide hormone genes in the mouse: somatostatin, glucagon, calcitonin, and parathyroid hormone.

作者信息

Lalley P A, Sakaguchi A Y, Eddy R L, Honey N H, Bell G I, Shen L P, Rutter W J, Jacobs J W, Heinrich G, Chin W W

出版信息

Cytogenet Cell Genet. 1987;44(2-3):92-7. doi: 10.1159/000132350.

Abstract

Mouse-Chinese hamster hybrids segregating mouse chromosomes were analyzed by Southern hybridization techniques to map the genes for somatostatin (Smst), glucagon (Gcg), calcitonin (Calc), and parathyroid hormone (Pth). The mouse gene for somatostatin, detected on a 20-kb EcoRI fragment, is located on mouse chromosome 16. Glucagon cDNA hybridized to a 14-kb EcoRI fragment residing on chromosome 2. Calcitonin and parathyroid hormone genes, detected on 7.8-kb HindIII and 6.0-kb BamHI fragments, respectively, were on mouse chromosome 7. The calcitonin and parathyroid hormone genes appear to be part of a larger linkage group which has been conserved in mouse and man.

摘要

通过Southern杂交技术分析了分离出小鼠染色体的小鼠-中国仓鼠杂种，以绘制生长抑素（Smst）、胰高血糖素（Gcg）、降钙素（Calc）和甲状旁腺激素（Pth）的基因图谱。在一个20kb的EcoRI片段上检测到的小鼠生长抑素基因位于小鼠16号染色体上。胰高血糖素cDNA与位于2号染色体上的一个14kb的EcoRI片段杂交。分别在7.8kb的HindIII片段和6.0kb的BamHI片段上检测到的降钙素基因和甲状旁腺激素基因位于小鼠7号染色体上。降钙素基因和甲状旁腺激素基因似乎是一个更大的连锁群的一部分，该连锁群在小鼠和人类中都得以保留。