Brazier J S, Citron D M, Goldstein E J
Anaerobe Reference Unit, Public Health Laboratory, Luton, UK.
J Appl Bacteriol. 1991 Oct;71(4):343-6. doi: 10.1111/j.1365-2672.1991.tb03798.x.
A new selective medium (JVN) for the isolation of Fusobacterium spp. from clinical material is described. The medium incorporates josamycin, vancomycin and norfloxacin (at 3, 4 and 1 microgram/ml, respectively) as the selective agents, plus 5% defibrinated horse blood in Fastidious Anaerobe Agar Base (Lab M). This formula allowed luxuriant growth of all 82 strains (eight recognized species) of fusobacteria tested, while significantly inhibiting 51/51 (100%) strains of facultative anaerobes and 45/51 (88%) strains of other obligate anaerobes. JVN medium allowed the successful isolation of strains of Fusobacterium naviforme, F. nucleatum and F. necrophorum from the gingivae of 9/16 healthy volunteers, and strains of F. varium and F. mortiferum from faecal suspensions seeded with these organisms.
本文描述了一种用于从临床样本中分离梭杆菌属细菌的新型选择性培养基（JVN）。该培养基含有交沙霉素、万古霉素和诺氟沙星（分别为3、4和1微克/毫升）作为选择剂，以及在苛求厌氧菌琼脂基础培养基（Lab M）中添加5%去纤维蛋白马血。该配方使所测试的82株梭杆菌（8个公认的菌种）均能旺盛生长，同时显著抑制了51/51（100%）的兼性厌氧菌菌株和45/51（88%）的其他专性厌氧菌菌株。JVN培养基成功地从9/16名健康志愿者的牙龈中分离出舟形梭杆菌、具核梭杆菌和坏死梭杆菌菌株，以及从接种这些细菌的粪便悬液中分离出多变梭杆菌和死亡梭杆菌菌株。
