Sutter V L, Sugihara P T, Finegold S M
Appl Microbiol. 1971 Nov;22(5):777-80. doi: 10.1128/am.22.5.777-780.1971.
A selective medium which allows detection of relatively small numbers of Fusobacterium varium in fecal specimens is described. Blood-agar containing 50 mug of rifampin per ml inhibits the growth of many species of Bacteriodes and of F. fusi-forme/nucleatum but allows good growth of F. varium and most strains of F. mortiferum. Quantitative cultures of 11 fecal specimens were done on rifampin and other selective and nonselective media. F. varium was recovered in counts of 10(6) and 10(7) per gram from two specimens on rifampin only. A third specimen yielded 10(10)F. varium on several media, including rifampin. Some Eubacterium and Clostridium species also grew on rifampin, and these ordinarily were distinguished from the Fusobacterium by colony morphology. This medium is of value in fecal flora studies and should be useful with other kinds of specimens where mixtures of organisms are common.
本文描述了一种选择性培养基，可用于检测粪便标本中数量相对较少的多变梭杆菌。每毫升含50微克利福平的血琼脂能抑制许多拟杆菌属菌种以及具核梭杆菌/梭形梭杆菌的生长，但能使多变梭杆菌和大多数死亡梭杆菌菌株良好生长。在利福平和其他选择性及非选择性培养基上对11份粪便标本进行了定量培养。仅在利福平培养基上，从两份标本中分别以每克10⁶和10⁷的数量回收了多变梭杆菌。在包括利福平在内的几种培养基上，第三份标本产生了10¹⁰个多变梭杆菌。一些真杆菌属和梭菌属菌种也能在利福平培养基上生长，通常可通过菌落形态将它们与梭杆菌属区分开来。这种培养基在粪便菌群研究中具有价值，在其他常见生物混合的标本类型中也应有用。
