一种纯化人外周血单核细胞的简单方法。Sepracell-MN的替代品。
A simple method for the purification of human peripheral blood monocytes. A substitute for Sepracell-MN.
作者信息
Denholm E M, Wolber F M
机构信息
Division of Hematology, Albany Medical College, NY 12208-3479.
出版信息
J Immunol Methods. 1991 Nov 22;144(2):247-51. doi: 10.1016/0022-1759(91)90092-t.
Sepracell-MN has provided a simple, rapid means of isolating peripheral blood monocytes. However this product is no longer available. Consequentially we have developed a Percoll gradient which matches Sepracell-MN in simplicity and yield of monocytes. Using this Percoll gradient, an average of 7 x 10(6) monocytes with a purity of 83% were obtained from 30-40 ml of blood. These monocytes were at least 97% viable and responded to chemotactic stimuli in comparable numbers to those prepared using Sepracell-MN.
Sepracell-MN提供了一种简单、快速分离外周血单核细胞的方法。然而，该产品已不再供应。因此，我们开发了一种Percoll梯度，其在单核细胞分离的简便性和产量方面与Sepracell-MN相当。使用这种Percoll梯度，从30 - 40毫升血液中平均可获得7×10⁶个单核细胞，纯度为83%。这些单核细胞的存活率至少为97%，并且对趋化刺激的反应数量与使用Sepracell-MN制备的单核细胞相当。
Zotero 插件