Morimoto Yuya, Tan Wei-heong, Tsuda Yukiko, Takeuchi Shoji
Center for International Research on Micromechatronics (CIRMM), Institute of Industrial Science (IIS), The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505, Japan.
Lab Chip. 2009 Aug 7;9(15):2217-23. doi: 10.1039/b900035f. Epub 2009 May 11.
We present a method for forming monodisperse semi-permeable microcapsules composed of an alginate-poly-L-lysine (PLL) membrane for the observation of encapsulated cells. These microcapsules were prepared with a monolithic three-dimensional microfluidic axisymmetric flow-focusing device by an internal gelation method using glucono-1,5-lactone in order to provide mild conditions for the cells. The microcapsules were sufficiently monodisperse and robust to be trapped in a bead-based microfluidic array system for easy observation. We also confirmed that (i) the alginate-PLL membrane is semi-permeable so that cells and microorganisms cannot pass through it but nutrients and wastes can, (ii) cells are able to move freely inside the semi-permeable microcapsules, and (iii) cells can be successfully proliferated in the microcapsules.
我们提出了一种形成由藻酸盐-聚-L-赖氨酸（PLL）膜组成的单分散半透性微胶囊的方法，用于观察被包裹的细胞。这些微胶囊是使用整体式三维微流控轴对称流动聚焦装置，通过采用葡萄糖酸-1,5-内酯的内部凝胶化方法制备的，以便为细胞提供温和的条件。这些微胶囊具有足够的单分散性和坚固性，能够被困在基于珠子的微流控阵列系统中以便于观察。我们还证实了：（i）藻酸盐-PLL膜是半透性的，因此细胞和微生物无法穿过，但营养物质和废物可以；（ii）细胞能够在半透性微胶囊内自由移动；（iii）细胞能够在微胶囊中成功增殖。
