Rushton B, Sharp J M
J Pathol. 1977 Mar;121(3):163-7. doi: 10.1002/path.1711210306.
Seven lambs were challenged with an aerosol of OA4 virus. Four developed mild pulmonary oedema and a sparse accumulation of mononuclear cells around blood vessels similar to results reported previously. In addition, three had focal hepatic necrosis and one of these three had a non-suppurative occlusive cholangitis and portal tract lymphangitis and thrombosis. Basophilic intranuclear IB's were identified in many necrotic hepatocytes, in lymphatic endothelial cells involved in thrombi but in only one bile duct epithelial cell. Ultrastructurally, virions with the characteristics of adenovirus were seen in hepatocytes. Although only one IB was seen in the affected biliary system there was no evidence that it was caused by an agent other than OA4. Virus was considered to infect the respiratory system directly, be swallowed to infect the alimentary system and carried, possibly within cells, via a haematogeneous and/or lymphogenous route to the liver. The effect of naturally acquired virus on sheep is unknown but it is possible that it may predispose the host to more serious infections.
七只羔羊接受了OA4病毒气溶胶的攻击。四只出现轻度肺水肿，血管周围有少量单核细胞积聚，这与先前报道的结果相似。此外，三只出现局灶性肝坏死，其中一只出现非化脓性闭塞性胆管炎、门静脉周围淋巴管炎和血栓形成。在许多坏死的肝细胞、血栓中的淋巴管内皮细胞中发现了嗜碱性核内包涵体，但仅在一个胆管上皮细胞中发现。超微结构上，在肝细胞中可见具有腺病毒特征的病毒粒子。虽然在受影响的胆道系统中仅发现一个包涵体，但没有证据表明它是由OA4以外的病原体引起的。病毒被认为直接感染呼吸系统，被吞咽后感染消化系统，并可能通过血行和/或淋巴途径在细胞内携带至肝脏。自然感染病毒对绵羊的影响尚不清楚，但有可能使宿主易患更严重的感染。
