Juranić Zorica D, Besu Irina, Jelić Svetislav, Konić-Ristić Aleksandra, Matković Suzana, Janković Ljiljana, Gavrilović Dušica, Radojčić Branka, Minić Ivana
Institute of Oncology and Radiology of Serbia, Pasterova 14, 11000, Belgrade, Serbia.
Faculty of Pharmacy, University of Belgrade, Belgrade, Serbia.
Int J Hematol. 2009 Sep;90(2):212-216. doi: 10.1007/s12185-009-0383-5. Epub 2009 Jul 17.
Non-Hodgkin lymphoma (NHL) represents heterogeneous group of diseases either B, or T cell origin. In order to assess whether food antigens contribute to the imbalance of immune response, the aim of this work was screening the sera of patients with (mostly) B cell NHL, and of people with non-malignant health disorders (NMD), as well as of healthy people for their immunoreactivity to food constituent gliadin, and to cow's milk proteins. Data obtained by ELISA tests show the existence of the enhanced immunoreactivity to food antigens in some NHL patients, as well as in some people with NMD. The high degree of coincidence in the presence of enhanced levels of immune complexes in circulation (CIC) and of immunoreactivity with gliadin in immunofixation (after the serum protein electrophoresis in agarose gel in veronal buffer, at pH 8.6) especially in NHL patients points that some antigliadin immunoreactivity unrevealed in ELISA tests could be hidden in CIC. This, only in the presence of malignant genotype, as well as the enhanced levels of CIC in some of NHL patients could both, at least partially contribute to the persistent non-specific support of disease. They call for the new research of the clinical importance of both, the elevated humoral immunity to food antigens (gluten, cow's milk proteins) for the course of this very severe hematological disease.
非霍奇金淋巴瘤（NHL）是一组起源于B细胞或T细胞的异质性疾病。为了评估食物抗原是否会导致免疫反应失衡，本研究旨在筛查（主要为）B细胞NHL患者、患有非恶性健康疾病（NMD）的人群以及健康人群的血清，检测其对食物成分麦醇溶蛋白和牛奶蛋白的免疫反应性。通过酶联免疫吸附测定（ELISA）试验获得的数据表明，一些NHL患者以及一些患有NMD的人群中存在对食物抗原增强的免疫反应性。循环免疫复合物（CIC）水平升高与免疫固定法中与麦醇溶蛋白的免疫反应性增强（在pH 8.6的巴比妥缓冲液中于琼脂糖凝胶上进行血清蛋白电泳后）高度吻合，尤其是在NHL患者中，这表明ELISA试验中未检测到的一些抗麦醇溶蛋白免疫反应性可能隐藏在CIC中。这一点，仅在存在恶性基因型的情况下，以及一些NHL患者中CIC水平升高，都可能至少部分导致疾病的持续非特异性支持。它们呼吁针对这种非常严重的血液疾病过程中，对食物抗原（麸质、牛奶蛋白）的体液免疫升高的临床重要性展开新的研究。
