CSIRO Livestock Industries, Queensland Bioscience Precinct, St. Lucia 4072, Queensland, Australia.
J Biomed Mater Res A. 2010 May;93(2):687-95. doi: 10.1002/jbm.a.32572.
Tissue adhesives and sealants are commonly used in surgery either as an adjunct to, or replacement for, sutures. Previously, we have shown that fibrinogen can be crosslinked rapidly to give a high-strength bond in the presence of a ruthenium(II) complex, a persulfate and irradiation with visible light, and that the crosslinked fibrinogen is nontoxic to cells in vitro. This approach addresses limitations to current fibrin sealants that typically have relatively slow curing times and low bond strengths. In the present study, we have evaluated the efficacy and safety of this new biological scaffold sealant in various animal models. When placed as solid implants into rats, the crosslinked fibrinogen persisted for at least 8 weeks but was fully resorbed by 18 weeks with minimal inflammatory responses. When used as a tissue adhesive for repair of skin incisions in rats or as an arterial haemostat in pig, the photo-crosslinked fibrinogen sealed tissue or arrested bleeding within 20 s of application. For the skin incisions, the fibrinogen sealant promoted rapid tissue vascularization and cellular infiltration with no adverse foreign body cell generation. New collagen deposition occurred and with time the matrix had remodelled to acquire large mature collagen fiber bundles which were accompanied by maximum regenerated tensile strength. This biomaterial system may find useful applications in surgical procedures where rapid curing and/or high strength tissue sealing is required.
组织黏合剂和密封剂在外科手术中通常被用作缝线的辅助或替代物。之前，我们已经证明在钌 (II) 配合物、过硫酸盐和可见光照射存在的情况下，纤维蛋白原可以快速交联以形成高强度的键，并且交联的纤维蛋白原对体外细胞是无毒的。这种方法解决了当前纤维蛋白密封剂的局限性，这些密封剂通常具有相对较慢的固化时间和低的键强度。在本研究中，我们在各种动物模型中评估了这种新型生物支架密封剂的功效和安全性。当作为固体植入物放置在大鼠中时，交联的纤维蛋白原至少持续 8 周，但在 18 周时被完全吸收，炎症反应最小。当用作大鼠皮肤切口的组织黏合剂或猪的动脉止血剂时，光交联的纤维蛋白原在应用后 20 秒内即可密封组织或止血。对于皮肤切口，纤维蛋白原密封剂促进了快速的组织血管化和细胞浸润，没有产生不良的异物细胞。新的胶原蛋白沉积发生，随着时间的推移，基质重塑以获得大的成熟胶原纤维束，伴随着最大的再生拉伸强度。这种生物材料系统可能在需要快速固化和/或高强度组织密封的手术中具有有用的应用。
