Yoshiyama Mikio, Kimura Kiyoshi
Honeybee Research Group, Animal Breeding and Reproduction Research Team, National Institute of Livestock and Grassland Science, 2 Ikenodai, Tsukuba, Ibaraki 305-0901, Japan.
J Invertebr Pathol. 2009 Oct;102(2):91-6. doi: 10.1016/j.jip.2009.07.005. Epub 2009 Jul 17.
We assessed the complexity of bacterial communities occurring in the digestive tract of the Japanese honeybee, Apis cerana japonica, using histological and 16S rRNA gene sequence analyzes. Both Gram-positive and -negative bacteria were observed, and the number of gut bacteria was higher in old larvae compared with young larvae. A total of 35 clones were obtained by a culture-dependent method, and 16S rRNA gene sequence analysis revealed that the bacterial population in the gut of Japanese honeybee was diverse, including the phyla firmicutes, actinobacteria, and alpha-, beta-, and gammaproteobacteria. Further investigation by in vitro inhibition assays was carried out to determine the ability of an isolate to inhibit Paenibacillus larvae, the causal agent of American foulbrood. Out of 35 isolates, seven showed strong inhibitory activity against P. larvae. Most of the antagonistic bacteria belonged to Bacillus species, suggesting that the bacterial isolates obtained in this study appear to be potential candidates for the biological control of P. larvae.
我们通过组织学和16S rRNA基因序列分析，评估了日本蜜蜂（Apis cerana japonica）消化道中细菌群落的复杂性。观察到了革兰氏阳性菌和革兰氏阴性菌，与幼虫相比，老龄幼虫肠道细菌数量更多。通过依赖培养的方法共获得35个克隆，16S rRNA基因序列分析表明，日本蜜蜂肠道中的细菌种群具有多样性，包括厚壁菌门、放线菌门以及α-、β-和γ-变形菌门。通过体外抑制试验进行了进一步研究，以确定一种分离物抑制美洲幼虫腐臭病病原体幼虫芽孢杆菌（Paenibacillus larvae）的能力。在35个分离物中，有7个对幼虫芽孢杆菌表现出强烈的抑制活性。大多数拮抗细菌属于芽孢杆菌属，这表明本研究中获得的细菌分离物似乎是幼虫芽孢杆菌生物防治的潜在候选者。
