Ogunremi Dele, Dupras Andrée Ann, Naushad Sohail, Gao Ruimin, Duceppe Marc-Olivier, Omidi Katayoun, Márquez Imelda Galván, Huang Hongsheng, Goodridge Lawrence, Lévesque Roger C, Hasan Nur A, Dadlani Manoj, Dixon Brent, Magierowski Sebastian, Masson Luke
Ottawa Laboratory Fallowfield, Canadian Food Inspection Agency, Ottawa, ON, Canada.
Department of Food Science and Agricultural Chemistry, McGill University, Sainte-Anne-de-Bellevue, QC, Canada.
Front Microbiol. 2020 Apr 17;11:602. doi: 10.3389/fmicb.2020.00602. eCollection 2020.
The rapid detection of foodborne microbial pathogens contaminating fresh fruits and vegetables during the intervening period between harvest and consumption could revolutionize microbial quality assurance of food usually consumed raw and those with a limited shelf life. We have developed a sensitive, shotgun whole genome sequencing protocol capable of detecting as few as 1 colony forming unit (cfu) of serovar Typhimurium spiked on 25 g of lettuce. The Ion Torrent sequencing platform was used to generate reads of globally amplified DNA from microbes recovered from the surface of lettuce followed by bioinformatic analyses of the nucleotide sequences to detect the presence of . The test is rapid and sensitive, and appropriate for testing perishable foods, and those consumed raw, for contamination. The test has the potential to be universally applicable to any microbial contaminant on lettuce as long as a suitable bioinformatics pipeline is available and validated. A universal test is expected to pave the way for preventive and precision food safety and the re-shaping of the entire spectrum of food safety investigations from the current disease-limiting, reactive procedure to a proactive, disease prevention process.
在新鲜水果和蔬菜收获后至食用前的这段时间内，快速检测其中污染的食源微生物病原体，可能会彻底改变通常生食的食品以及保质期有限的食品的微生物质量保证。我们已经开发出一种灵敏的鸟枪法全基因组测序方案，能够检测出接种在25克生菜上低至1个鼠伤寒血清型菌落形成单位（cfu）。使用离子激流测序平台从生菜表面回收的微生物中生成全局扩增DNA的读数，随后对核苷酸序列进行生物信息学分析以检测[此处原文缺失具体检测对象]的存在。该检测快速且灵敏，适用于检测易腐食品以及生食食品是否受到[此处原文缺失具体检测对象]污染。只要有合适的生物信息学流程并经过验证，该检测就有可能普遍适用于生菜上的任何微生物污染物。一种通用检测有望为预防性和精准食品安全铺平道路，并将食品安全调查的整个范围从当前的疾病限制、被动程序重塑为主动的疾病预防过程。
