Harikrishnan Ramasamy, Balasundaram Chellam, Kim Man-Chul, Kim Ju-Sang, Han Yong-Jae, Heo Moon-Soo
Marine Applied Microbes and Aquatic Organism Disease Control Lab, Department of Aquatic Biomedical Sciences, School of Marine Biomedical Sciences, College of Ocean Science & Marine and Environmental Research Institute, Jeju National University, Jeju 690-756, Republic of Korea.
Fish Shellfish Immunol. 2009 Sep;27(3):508-15. doi: 10.1016/j.fsi.2009.07.004. Epub 2009 Jul 17.
This study reports the effect of aqueous, ethanol and methanol triherbal solvent extract from Azadirachta indica, Ocimum sanctum and Curcuma longa on innate immune mechanisms such as phagocytosis activity, respiratory burst activity, alternative complement activity and lysozyme activity and disease resistance in goldfish (Carassius auratus) against Aeromonas hydrophila. Fish were intraperitoneally injected with different doses of 0, 5, 50 and 100 mg kg(-1) body weight of each triherbal solvent extracts. The functional immunity in terms of percentage mortality and Relative Percent Survival (RPS) and innate immune response was assessed on week 1, 2 and 4 by challenging with live A. hydrophila (1 x 10(7) cells ml(-1)). All the chosen innate immune parameters were enhanced in the ethanol and methanol triherbal solvent extract treatment after week 2. However, the aqueous triherbal extract was enhanced only after week 4. The ethanol and methanol triherbal solvent extracts administration preceding the challenge with live A. hydrophila decreased the percentage mortality in the experimental groups with the consequence increase in RPS values. The study indicates that all the doses of ethanol or methanol triberbal solvent extracts could be positively influence the immune response and protect the heath status of goldfish against A. hydrophila infection.
本研究报告了印楝、圣罗勒和姜黄的水、乙醇和甲醇三种草药溶剂提取物对金鱼（Carassius auratus）先天免疫机制（如吞噬活性、呼吸爆发活性、替代补体活性和溶菌酶活性）以及对嗜水气单胞菌抗病能力的影响。将不同剂量（0、5、50和100 mg kg(-1)体重）的每种三种草药溶剂提取物腹腔注射到鱼体内。在第1、2和4周，通过用活的嗜水气单胞菌（1 x 10(7) 个细胞/ml）攻击来评估以死亡率百分比和相对存活率（RPS）表示的功能免疫力以及先天免疫反应。在第2周后，乙醇和甲醇三种草药溶剂提取物处理组中所有选定的先天免疫参数均有所增强。然而，水三种草药提取物仅在第4周后增强。在用活的嗜水气单胞菌攻击之前给予乙醇和甲醇三种草药溶剂提取物可降低实验组的死亡率百分比，从而使RPS值增加。该研究表明，所有剂量的乙醇或甲醇三种草药溶剂提取物均可对免疫反应产生积极影响，并保护金鱼的健康状况免受嗜水气单胞菌感染。
