Kocyigit Yukse, Atamer Yildiz, Uysal Ersin
Department of Physiology, Medical Faculty, Dicle University Diyarbakir, Turkey.
Saudi Med J. 2009 Jul;30(7):893-6.
To investigate the effect of oral treatment of Wistar albino rats with different doses of Nigella sativa L. (NS) powdered seeds on the levels of serum lipids.
This study was performed in the Medical Science Application and Research Center of Dicle University, Diyarbakir, Turkey, from February 2003 to December 2008. A total of 75 Wistar albino male rats, 60 of them with NS supplementation and 15 animals acting as controls, were included in the study. The NS groups were divided into 4 main groups of 15 each. Four doses of NS were used (100, 200, 400, and 600 mg/kg/day). Each dose group was further divided into 3 duration subgroups of 5 rats each, the feeding of NS seeds continued for one, 2, and 4 weeks. Control animals were divided into 3 main groups of 5 rats each. The rats were sacrificed at one, 2, and 4 weeks after feeding. Lipid parameters were measured.
Rats treated with the 400mg dose for one week's duration showed a significant increase in high-density lipoprotein-cholesterol levels. There was a significant decrease in low density lipoprotein-cholesterol levels after one week for 400 and 600 mg doses, and all doses after 2 weeks and 4 weeks for 200 and 600 mg doses when compared to control groups. There was a significant decrease in very low-density lipoprotein-cholesterol levels after one week for 200, 400, and 600 mg doses, and all doses for 2 and 4 weeks. A 400 mg dose for 2 weeks, and all doses for 4 weeks caused a significant decrease in triglyceride levels. There was a significant decrease of total cholesterol levels in all doses after 4 weeks of NS feeding.
These results indicate that NS may ameliorate the alteration in the lipid levels caused by diseases or toxic agents.
研究不同剂量的黑种草籽粉口服给药对Wistar白化大鼠血脂水平的影响。
本研究于2003年2月至2008年12月在土耳其迪亚巴克尔的狄克莱大学医学科学应用与研究中心进行。共有75只Wistar白化雄性大鼠纳入研究，其中60只给予黑种草籽补充剂，15只作为对照。黑种草籽组分为4个主要组，每组15只。使用了4种剂量的黑种草籽（100、200、400和600毫克/千克/天）。每个剂量组进一步分为3个持续时间亚组，每组5只大鼠，黑种草籽喂养持续1、2和4周。对照动物分为3个主要组，每组5只大鼠。在喂养后1、2和4周处死大鼠，测量血脂参数。
给予400毫克剂量持续1周的大鼠高密度脂蛋白胆固醇水平显著升高。与对照组相比，400毫克和600毫克剂量在1周后低密度脂蛋白胆固醇水平显著降低，200毫克和600毫克剂量在2周和4周后所有剂量均显著降低。200、400和600毫克剂量在1周后极低密度脂蛋白胆固醇水平显著降低，2周和4周后所有剂量均显著降低。400毫克剂量持续2周以及所有剂量持续4周导致甘油三酯水平显著降低。黑种草籽喂养4周后所有剂量的总胆固醇水平均显著降低。
这些结果表明，黑种草籽可能改善由疾病或有毒物质引起的血脂水平变化。
