Lokhande Vinayak H, Kudale Subhash, Nikalje Ganesh, Desai Neetin, Suprasanna Penna
Shri Shiv Chhatrapati College of Arts, Commerce and Science, Bodkenagar, Junnar, Pune 410 502, India.
School of Biotechnology and Bioinformatics, Dr. D. Y. Patil University, C. B. D. Belapur, Navi Mumbai 400 614, India.
Biotechnol Rep (Amst). 2015 Aug 28;8:56-63. doi: 10.1016/j.btre.2015.08.002. eCollection 2015 Dec.
In this study, we report phytoremediation of textile dyes using hairy roots derived through (NCIM 5140) infection of in vitro leaf and stem explants of a halophyte (L.) L. Leaf explants showed higher frequency of hairy root induction (70%) than stem explants (30%), and maximum number of roots (leaf 42.3 ± 2.4 and stem 50.3 ± 1.7). Transformed nature of hairy roots was ascertained by amplifying 970 bp region of T-DNA of Ri plasmid. Hairy roots were screened for phytoremediation of various textile dyes and results showed that HRs were able to degrade Reactive green 19A HE4BD upto 98% within 5 days of incubation. Spectrophotometric analysis showed decrease in dye concentration while HPLC and FTIR analysis confirmed its degradation. Seed germination assay demonstrated non-toxic nature of the extracted metabolites. This is the first report on induction of hairy root culture in and phytoremediation of textile dyes.
在本研究中，我们报道了利用通过发根农杆菌（NCIM 5140）感染盐生植物滨藜（L.）L.的离体叶片和茎段外植体所获得的毛状根对纺织染料进行植物修复。叶片外植体的毛状根诱导频率（70%）高于茎段外植体（30%），且根的数量最多（叶片为42.3±2.4，茎段为50.3±1.7）。通过扩增Ri质粒T-DNA的970 bp区域确定了毛状根的转化性质。对毛状根进行了各种纺织染料植物修复筛选，结果表明，毛状根在培养5天内能够将活性绿19A HE4BD降解高达98%。分光光度分析表明染料浓度降低，而高效液相色谱和傅里叶变换红外光谱分析证实了其降解。种子发芽试验证明了提取代谢物的无毒性质。这是关于滨藜毛状根培养诱导及纺织染料植物修复的首次报道。
