College of Materials Science and Engineering, South China University of Technology, Guangzhou 510640, PR China.
J Biomater Sci Polym Ed. 2009;20(11):1555-65. doi: 10.1163/092050609X12464345036768.
A chitosan (CS)/hydroxyapatite (HAP) nanohybrid scaffold with high porosity and homogeneous nanostructure was fabricated through a bionic treatment combined with thermally-induced phase separation. The nano-HAP particles were formed in situ in the scaffold at room temperature instead of mechanically mixing the powders with the polymer component. The scaffold was macroporous with a pore size of about 100-136 microm. The nano-sized HAP particles with diameters of 90-200 nm were scattered homogeneously in the interactively connective pores. Both the improvement of the compressive modulus and yield strength of the scaffolds showed that the in situ nano-HAP particles reinforced the microstructure of the scaffold. The in vitro bioactivity study carried out in simulated body fluid (SBF) indicated good mineralization activity. The crystallization phenomenon suggested that the nano-HAP particles have positive impacts on directing apatite crystallization in the scaffold and led to the good bioactivity of the nanohybrid scaffold.
通过仿生处理与热致相分离相结合，制备了一种具有高孔隙率和均匀纳米结构的壳聚糖（CS）/羟基磷灰石（HAP）纳米杂化支架。纳米 HAP 颗粒在室温下原位形成于支架中，而不是将粉末与聚合物成分机械混合。支架具有约 100-136 微米的大孔尺寸。直径为 90-200nm 的纳米级 HAP 颗粒均匀分散在相互连接的孔中。支架压缩模量和屈服强度的提高均表明，原位纳米 HAP 颗粒增强了支架的微观结构。在模拟体液（SBF）中进行的体外生物活性研究表明具有良好的矿化活性。结晶现象表明纳米 HAP 颗粒对引导支架中磷灰石结晶具有积极影响，导致纳米杂化支架具有良好的生物活性。
