Martins Manoela Domingues, Fernandes Kristianne Porta Santos, Motta Lara Jansinski, Santos Elaine Marcilio, Pavesi Vanessa Christina Santos, Bussadori Sandra Kalil
Nove de Julho University, São Paulo, SP, Brazil.
J Dent Child (Chic). 2009 May-Aug;76(2):123-9.
Chemomechanical caries removal allies an atraumatic technique with antimicrobiotic characteristics, minimizing painful stimuli and maximally preserving healthy dental structures. The purpose of this study was to compare the cytotoxic effects of papain-based gel (Papacarie) and another caries-removing substance, Carisolv, to a nontreatment control on cultured fibroblasts in vitro and the biocompatibility in subcutaneous tissue in vivo. The cytotoxicity analysis was performed on fibroblast cultures (NIH-3T3) after 0-, 4-, 8-, and 12-hour exposure (cell viability assay) and after 1-, 3-, 5-, and 7-day exposure (survival assay). In the in vivo study, the 2 compounds were introduced into polyethylene tubes that were implanted into subcutaneous tissues of rats. After 1, 7, 14, 30, and 60 days, tissue samples were examined histologically. Cell viability did not differ between the 2 experimental groups. The control group, however, showed significantly higher percentage viability. There were no differences in cell survival between the control and experimental groups. The histological analysis revealed a moderate inflammatory response at 2 and 7 days and a mild response at 15 days, becoming almost imperceptible by 30 and 60 days in both experimental groups. The 2 tested substances exhibited acceptable biocompatibilities and demonstrated similar responses in the in vitro cytotoxicity and in vivo implantation assay.
化学机械性龋损去除术将一种无创伤技术与抗菌特性相结合，最大限度地减少疼痛刺激，并最大程度地保留健康的牙齿结构。本研究的目的是比较基于木瓜蛋白酶的凝胶（Papacarie）和另一种龋损去除物质Carisolv与非治疗对照组在体外培养的成纤维细胞上的细胞毒性作用，以及在体内皮下组织中的生物相容性。在0小时、4小时、8小时和12小时暴露后（细胞活力测定）以及1天、3天、5天和7天暴露后（存活测定），对成纤维细胞培养物（NIH-3T3）进行细胞毒性分析。在体内研究中，将这两种化合物引入植入大鼠皮下组织的聚乙烯管中。在1天、7天、14天、30天和60天后，对组织样本进行组织学检查。两个实验组之间的细胞活力没有差异。然而，对照组的活力百分比明显更高。对照组和实验组之间的细胞存活没有差异。组织学分析显示，在第2天和第7天有中度炎症反应，在第15天有轻度反应，在两个实验组中到第30天和第60天几乎难以察觉。这两种受试物质表现出可接受的生物相容性，并且在体外细胞毒性和体内植入试验中表现出相似的反应。
