Zand Vahid, Mokhtari Hadi, Lotfi Mehrdad, Rahimi Saeed, Sohrabi Aydin, Badamchi Zadeh Sina, Mojaver Kahnamooie Hanieh, Tehranchi Pardis
Dental and Periodontal Research Center, Department of Endodontics, Dental School, Tabriz University of Medical Sciences, Tabriz, Iran;
Department of Endodontics, Dental School, Tabriz University of Medical Sciences, Tabriz, Iran;
Iran Endod J. 2014 Spring;9(2):131-6. Epub 2014 Mar 8.
The aim of this in vitro study was to evaluate the effect of an experimental irrigation solution, containing two different concentrations of papain, Tween 80, 2% chlorhexidine and EDTA, on removal of the smear layer.
Thirty-six single-rooted teeth were divided into two experimental groups (n=12) and two positive and negative control groups of six. The canals were prepared with BioRaCe instruments up to BR7 (60/0.02). In group 1, canals were irrigated with a combination of 1% papain, 17% EDTA, Tween 80 and 2% CHX; in group 2, canals were irrigated with a combination of 0.1% papain, 17% EDTA, Tween 80 and 2% CHX. In group 3 (the negative control), the canal was irrigated with 2.5% NaOCl during instrumentation and at the end of preparation with 1 mL of 17% EDTA was used; in group 4 (positive control), normal saline was used for irrigation. The amount of the remaining smear layer was quantified according to Hulsmann method using scanning electron microscopy (SEM). Data was analyzed by the Kruskal-Wallis and Mann-Whitney tests.
Two-by-two comparisons of the groups revealed no significant differences in terms of smear layer removal at different canal sections between the negative control group (standard regiment for smear layer removal) and 1% papain groups (P<0.05).
Under the limitations of the present study, combination of 1% papain, EDTA, 2% chlorhexidine and Tween 80 can effectively remove smear layer from canal walls.
本体外研究的目的是评估一种实验性冲洗液对去除玷污层的效果，该冲洗液含有两种不同浓度的木瓜蛋白酶、吐温80、2%洗必泰和乙二胺四乙酸（EDTA）。
将36颗单根牙分为两个实验组（n = 12）以及两个阳性和阴性对照组，每组6颗。使用BioRaCe器械将根管预备至BR7（60/0.02）。第1组，根管用1%木瓜蛋白酶、17% EDTA、吐温80和2%洗必泰的混合液冲洗；第2组，根管用0.1%木瓜蛋白酶、17% EDTA、吐温80和2%洗必泰的混合液冲洗。第3组（阴性对照组），在器械预备过程中用2.5%次氯酸钠冲洗根管，预备结束时使用1 mL 17% EDTA；第4组（阳性对照组），用生理盐水冲洗。使用扫描电子显微镜（SEM）根据胡尔斯曼方法对剩余玷污层的量进行定量。数据采用克鲁斯卡尔 - 沃利斯检验和曼 - 惠特尼检验进行分析。
各组两两比较显示，阴性对照组（去除玷污层的标准方案）和1%木瓜蛋白酶组在不同根管节段的玷污层去除方面无显著差异（P<0.05）。
在本研究的局限性下，1%木瓜蛋白酶、EDTA、2%洗必泰和吐温80的组合可有效去除根管壁上的玷污层。
