Institute of Biochemical Plant Pathology, Helmholtz Zentrum München, German Research Center for Environmental Health, D-85764 Neuherberg, Germany.
J Proteomics. 2009 Nov 2;73(1):1-9. doi: 10.1016/j.jprot.2009.07.002. Epub 2009 Jul 17.
During the last two decades nitric oxide (NO) has emerged as a new chemical messenger in plant biology, which is involved in many different physiological processes, such as plant defense, transpiration and gas exchange, seed germination, and root development. Protein S-nitrosylation, the post-translational modification of thiol residues, has been suggested to be the most important mechanism for transduction of the bioactivity of NO. The characterization of protein S-nitrosylation as well as the physiological relevance of this type of modification is essential information, which is necessary to understand the function of NO in plants. In this review we focus on the formation of nitrosothiols and describe the chemistry of NO and thiol groups. Furthermore, different methods for detection of S-nitrosothiols are highlighted and the function of S-nitrosylation in plants is discussed.
在过去的二十年中，一氧化氮（NO）作为植物生物学中的一种新的化学信使出现，它参与了许多不同的生理过程，如植物防御、蒸腾和气体交换、种子萌发和根系发育。蛋白质的 S-亚硝基化，即巯基残基的翻译后修饰，被认为是传递 NO 生物活性的最重要机制。蛋白质 S-亚硝基化的特征以及这种修饰类型的生理学相关性是理解 NO 在植物中功能所必需的重要信息。在这篇综述中，我们重点介绍了亚硝硫醇的形成，并描述了 NO 和巯基基团的化学性质。此外，还强调了不同的 S-亚硝硫醇检测方法，并讨论了 S-亚硝基化在植物中的功能。
