Unit of Animal Infectious Diseases, National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, 1 Shizishan Street, Wuhan, Hubei 430070, PR China.
Vet Microbiol. 2010 Jan 6;140(1-2):171-5. doi: 10.1016/j.vetmic.2009.06.034. Epub 2009 Jun 30.
Streptococcus suis (S. suis) is a major swine pathogen and an emerging zoonotic agent. However, the current understanding of the S. suis pathogenesis of infection remains limited. In the present study, an IgA1 protease of S. suis was identified for the first time. The protein was recombinantly expressed in Escherichia coli, and the purified recombinant protein showed good cleaving reactivity to IgA1 and was highly immunoreactive to convalescent sera. The gene was found to exist in most pathogenic strains but scarcely in non-invasive isolates. These findings suggested that the IgA1 protease might play an important role in the pathogenesis of S. suis.
猪链球菌（S. suis）是一种主要的猪病原体，也是一种新兴的人畜共患病原体。然而，目前对于 S. suis 感染的发病机制的了解仍然有限。在本研究中，首次鉴定了一种猪链球菌的 IgA1 蛋白酶。该蛋白在大肠杆菌中得到了重组表达，纯化的重组蛋白对 IgA1 具有良好的切割反应性，并且对恢复期血清具有高度的免疫反应性。该基因存在于大多数致病性菌株中，但在非侵袭性分离株中几乎不存在。这些发现表明，IgA1 蛋白酶可能在 S. suis 的发病机制中发挥重要作用。
