Kang Hye Soon, Lee Soon Dong
Department of Science Education, Cheju National University, Jeju 690-756, Republic of Korea.
Int J Syst Evol Microbiol. 2009 Sep;59(Pt 9):2264-8. doi: 10.1099/ijs.0.008326-0. Epub 2009 Jul 20.
A Gram-negative, aerobic, orange-coloured bacterium, designated strain GSW-2T, was isolated from seawater sampled at a beach and its taxonomic status was established by using a polyphasic approach. Vegetative cells produced a prostheca and reproduced by budding. Mature vegetative cells were spherical, oval- or rod-shaped (0.6-1.1x1.2-1.3 microm) and both vegetative cells and buds produced one or more flagella. Cells grew well at 30 degrees C and at pH 8.1-9.1, and produced a non-diffusible pigment. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain GSW-2T was most closely related to the type strain of Hirschia baltica (97.5% similarity), a member of the family Hyphomonadaceae, class Alphaproteobacteria. Chemotaxonomic characteristics supported the assignment of strain GSW-2T to the genus Hirschia, including the dominant cellular fatty acids (summed feature 7 and C16:0), isoprenoid quinone (mainly Q-10) and DNA G+C content (44.5 mol%). Levels of DNA-DNA relatedness between strain GSW-2T and Hirschia baltica DSM 5838T were 8.8-13.6%. On the basis of phenotypic features and DNA-DNA hybridization data, strain GSW-2T is considered to represent a novel species of the genus Hirschia, for which the name Hirschia maritima sp. nov. is proposed. The type strain is GSW-2T (=DSM 19733T=JCM 14974T).
从海滩采集的海水中分离出一株革兰氏阴性、需氧、橙色细菌，命名为菌株GSW-2T，并采用多相分类法确定其分类地位。营养细胞产生柄并通过出芽进行繁殖。成熟的营养细胞呈球形、椭圆形或杆状（0.6-1.1×1.2-1.3微米），营养细胞和芽均产生一根或多根鞭毛。细胞在30℃和pH 8.1-9.1条件下生长良好，并产生一种不可扩散的色素。基于16S rRNA基因序列的系统发育分析表明，菌株GSW-2T与波罗的海希氏菌模式菌株关系最为密切（相似性为97.5%），波罗的海希氏菌属于α-变形菌纲生丝微菌科。化学分类特征支持将菌株GSW-2T归入希氏菌属，包括主要的细胞脂肪酸（特征性总和7和C16:0）、类异戊二烯醌（主要为Q-10）和DNA G+C含量（44.5摩尔%）。菌株GSW-2T与波罗的海希氏菌DSM 5838T之间的DNA-DNA相关性水平为8.8-13.6%。基于表型特征和DNA-DNA杂交数据，菌株GSW-2T被认为代表希氏菌属的一个新物种，为此提出新名称海洋希氏菌（Hirschia maritima sp. nov.）。模式菌株为GSW-2T（=DSM 19733T=JCM 14974T）。
