Division of Life Sciences, Korea Polar Research Institute, 26 Songdomirae-ro, Yeonsu-gu, Incheon, 21990, Republic of Korea.
Antonie Van Leeuwenhoek. 2024 Sep 25;118(1):10. doi: 10.1007/s10482-024-02021-4.
Gram-staining-negative, aerobic, white-cream-pearly colony, coccobacilli, and non-motile bacterial strain, PAMC 29798 was isolated from an Antarctic lichen. The strain was acidotolerant and psychrotolerant growing at pH 4.0-7.5 (optimally at pH 4.0-6.5) and 0-25 °C (optimally at 10-20 °C). The major fatty acids are Summed Feature 8, C 2OH, and C cyclo ω8c. The major respiratory quinone was Q-10. Phylogenetic and phylogenomic analyses indicated that strain PAMC 29798 belonged to the genus Acidisoma and 16S rRNA gene sequences of PAMC 29798 were closely related to Acidisoma silvae (97.7% sequence similarity), Acidisoma cellulosilyticum (96.5%), Acidisoma tundrae (96.5%), and Acidisoma sibiricum (96.3%). Genomic relatedness analyses showed that strain PAMC 29798 was clearly distinguished from type strains of the genus Acidisoma based on values of average nucleotide identity (< 75%) and the digital DNA-DNA hybridization (< 19.6%). Genome analysis revealed that the genome size of PAMC 29798 is approximately 5.0 Mb with a G+C content of 63.4%. The complete genome comprises 5 contigs containing 4636 protein-coding genes, 46 tRNA genes, and 2 rRNA operons. The genome possesses genes for light-harvesting complexes, type-II photosynthetic reaction center, and C-P lyase to solubilize organic phosphates, while genes encoding nitrogenase iron protein involved in the nitrogen fixation were not present. Based on the results of phylogenetic, genome-based relatedness, and physiological and genomic analyses, strain PAMC 29798 is proposed to represent a novel species of the genus Acidisoma, with the name Acidisoma cladoniae. The type strain is PAMC 29798 (= KCTC 82159 = JCM 35634).
一株分离自南极地衣的革兰氏染色阴性、需氧、白色奶油珍珠色菌落、球杆菌、不运动的细菌菌株 PAMC 29798。该菌株耐酸和耐寒，可在 pH 值为 4.0-7.5（最佳 pH 值为 4.0-6.5）和 0-25°C（最佳温度为 10-20°C）下生长。主要脂肪酸为 Summed Feature 8、C 2OH 和 C cyclo ω8c。主要呼吸醌为 Q-10。系统发育和系统基因组分析表明，菌株 PAMC 29798 属于 Acidisoma 属，其 16S rRNA 基因序列与 Acidisoma silvae（97.7%序列相似性）、Acidisoma cellulosilyticum（96.5%）、Acidisoma tundrae（96.5%）和 Acidisoma sibiricum（96.3%）密切相关。基因组相关性分析表明，菌株 PAMC 29798 与 Acidisoma 属的模式菌株在平均核苷酸同一性（<75%）和数字 DNA-DNA 杂交（<19.6%）值上明显不同。基因组分析表明，PAMC 29798 的基因组大小约为 5.0 Mb，GC 含量为 63.4%。完整的基因组由 5 个 contigs 组成，包含 4636 个蛋白编码基因、46 个 tRNA 基因和 2 个 rRNA 操纵子。基因组具有光收集复合物、II 型光合作用反应中心和 C-P 裂解酶的基因，可溶解有机磷酸盐，而固氮酶铁蛋白参与固氮的基因则不存在。基于系统发育、基于基因组的相关性以及生理和基因组分析的结果，菌株 PAMC 29798 被提议代表 Acidisoma 属的一个新种，命名为 Acidisoma cladoniae。模式菌株为 PAMC 29798（=KCTC 82159=JCM 35634）。
