Kim Min-Soo, Roh Seong Woon, Nam Young-Do, Chang Ho-Won, Kim Kyoung-Ho, Jung Mi-Ja, Choi Jung-Hye, Park Eun-Jin, Bae Jin-Woo
Department of Life and Nanopharmaceutical Sciences and Department of Biology, Kyung Hee University, Seoul 130-701, Republic of Korea.
Int J Syst Evol Microbiol. 2009 Sep;59(Pt 9):2313-6. doi: 10.1099/ijs.0.007260-0. Epub 2009 Jul 20.
A novel Gram-negative and facultative anaerobic strain, designated MS1T, was isolated from gajami sikhae, a traditional fermented food in Korea made from flatfish. Strain MS1T was motile, rod-shaped and oxidase- and catalase-positive, and required 1-2% (w/v) NaCl for growth. Growth occurred at temperatures ranging from 4 to 40 degrees C and the pH range for optimal growth was pH 6.5-9.0. Strain MS1T was capable of reducing trimethylamine oxide, nitrate and thiosulfate. Phylogenetic analysis placed strain MS1T within the genus Alishewanella. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain MS1T was related closely to Alishewanella aestuarii B11T (98.67% similarity) and Alishewanella fetalis CCUG 30811T (98.04% similarity). However, DNA-DNA reassociation experiments between strain MS1T and reference strains showed relatedness values<70% (42.6 and 14.8% with A. aestuarii B11T and A. fetalis CCUG 30811T, respectively). Genotypic, physiological and biochemical analyses allowed the differentiation of strain MS1T from type strains of species belonging to the genus Alishewanella. Therefore, we propose that strain MS1T (=KCTC 22429T=JCM 15561T) is assigned to a novel species, Alishewanella jeotgali sp. nov.
从韩国传统发酵食品——由比目鱼制成的加贾米西凯（gajami sikhae）中分离出了一株新型革兰氏阴性兼性厌氧菌株，命名为MS1T。菌株MS1T具有运动性，呈杆状，氧化酶和过氧化氢酶呈阳性，生长需要1-2%（w/v）的NaCl。在4至40摄氏度的温度范围内均可生长，最适生长pH范围为6.5-9.0。菌株MS1T能够还原氧化三甲胺、硝酸盐和硫代硫酸盐。系统发育分析将菌株MS1T归入阿利氏菌属（Alishewanella）。基于16S rRNA基因序列的系统发育分析表明，菌株MS1T与河口阿利氏菌（Alishewanella aestuarii）B11T（相似度为98.67%）和胎儿阿利氏菌（Alishewanella fetalis）CCUG 30811T（相似度为98.04%）密切相关。然而，菌株MS1T与参考菌株之间的DNA-DNA重结合实验显示相关性值<70%（与河口阿利氏菌B11T和胎儿阿利氏菌CCUG 30811T的相关性分别为42.6%和14.8%）。基因型、生理和生化分析使菌株MS1T能够与阿利氏菌属的模式菌株区分开来。因此，我们提议将菌株MS1T（=KCTC 22429T=JCM 15561T）归入一个新物种，即盐渍阿利氏菌（Alishewanella jeotgali）新种。
