S. N. Winogradsky Institute of Microbiology, Russian Academy of Sciences, Prospect 60-let Octyabrya 7/2, Moscow 117312, Russia.
Technical University Braunschweig, Spielmannstrasse 7, Braunschweig, Germany.
Int J Syst Evol Microbiol. 2012 Mar;62(Pt 3):638-646. doi: 10.1099/ijs.0.007005-0. Epub 2011 May 6.
Three strains of Gram-negative, aerobic, motile bacteria with bipolar flagella were isolated from acidic tundra wetland soils near the city of Vorkuta and from the Chukotka and Yugorsky Peninsulas and designated strains V-022(T), Ch-022 and Ju-022. The cells were rod-shaped, 0.5-0.6 µm in width and 1.3-4.5 µm in length and reproduced by irregular fission. These bacteria were facultative methylotrophs that used methanol, methylamines and a wide range of other sources of carbon and energy such as sugars and polysaccharides, ethanol and amino acids. The isolates used the Calvin-Benson pathway for the assimilation of one-carbon compounds and were unable to fix nitrogen. The new strains were moderately acidophilic and psychrotolerant, capable of growth over a pH range of 4.0 to 7.8, with optimum growth at pH 5.5-6.0. Growth occurred between 4 and 30 °C (optimum 20-25 °C). The principal phospholipid fatty acid was C(18:1)ω7c. The DNA G+C content of strain V-022(T) was 65.2 mol%. Analysis of the 16S rRNA gene sequences revealed that all three isolates V-022(T), Ch-022 and Yu-022 exhibited almost identical 16S rRNA gene sequences (99.9% gene sequence similarity) and formed a new lineage within the class Alphaproteobacteria. The name Methylorosula polaris is suggested to accommodate this new genus and novel species with strain V-022(T) (=DSM 22001(T)=VKM V-2485(T)) as the type strain of the type species.
三株革兰氏阴性、需氧、具有双极鞭毛的运动细菌从沃尔库塔市附近的酸性冻原生态湿地土壤以及楚科奇半岛和尤戈尔半岛分离得到，并分别命名为菌株 V-022(T)、Ch-022 和 Ju-022。细胞呈杆状，宽 0.5-0.6 µm，长 1.3-4.5 µm，通过不规则分裂繁殖。这些细菌是兼性甲醇营养菌，可利用甲醇、甲基胺和各种其他碳源和能源，如糖和多糖、乙醇和氨基酸。这些分离株利用卡尔文-本森途径同化一碳化合物，且不能固定氮。新菌株中度嗜酸且耐低温，可在 pH 值为 4.0 到 7.8 的范围内生长，最适生长 pH 值为 5.5-6.0。在 4 到 30°C（最适 20-25°C）的温度范围内生长。主要磷脂脂肪酸为 C(18:1)ω7c。菌株 V-022(T)的 DNA G+C 含量为 65.2 mol%。16S rRNA 基因序列分析表明，所有三株分离株 V-022(T)、Ch-022 和 Ju-022 的 16S rRNA 基因序列几乎完全相同（99.9%基因序列相似性），并在α变形菌纲内形成了一个新的谱系。建议使用 Methylorosula polaris 来容纳这个新属和新种，以菌株 V-022(T)（=DSM 22001(T)=VKM V-2485(T)）作为模式种的模式株。
