Wang Yong-Xia, Liu Ji-Hui, Zhang Xiao-Xia, Chen Yi-Guang, Wang Zhi-Gang, Chen Yun, Li Qin-Yuan, Peng Qian, Cui Xiao-Long
Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan 650091, PR China.
Int J Syst Evol Microbiol. 2009 Oct;59(Pt 10):2575-81. doi: 10.1099/ijs.0.009340-0. Epub 2009 Jul 21.
Two Gram-negatively stained, facultatively anaerobic, non-motile, vibrioid and rod-shaped, chemoheterotrophic bacterial strains, designated YIM D82(T) and YIM D812(T), were isolated from a salt mine in Yunnan, south-west China. DNA-DNA hybridization, genomic DNA G+C content and phylogenetic analyses based on 16S rRNA gene sequences divided the two isolates into two distinct genospecies that were also clearly differentiated by fatty acid profiles, carbon source utilization patterns, antibiotic susceptibility and biochemical characteristics. The two isolates grew in the presence of 1.5-20 % NaCl, and optimally at 28 degrees C and pH 7.5. The genomic DNA G+C contents of strains YIM D82(T) and YIM D812(T) were 61.5 and 62.3 mol%, respectively. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strains YIM D82(T) and YIM D812(T) were members of the family Rhodospirillaceae and showed 90.5-90.6 % and 90.1-90.2 % similarities with their closest relatives, Rhodovibrio sodomensis and Rhodovibrio salinarum, respectively. Differential phenotypic and genotypic characteristics of the two isolates from recognized genera showed that the two strains should be classified as representing a new genus and two novel species for which the names Fodinicurvata sediminis gen. nov., sp. nov. (type strain YIM D82(T)=DSM 21159(T)=KCTC 22351(T)) and Fodinicurvata fenggangensis sp. nov. (type strain YIM D812(T)=CCTCC AA 208037(T)=DSM 21160(T)) are proposed.
从中国西南部云南省的一个盐矿中分离出两株革兰氏阴性染色、兼性厌氧、无运动性、呈弧状和杆状的化能异养细菌菌株，分别命名为YIM D82(T)和YIM D812(T)。基于16S rRNA基因序列的DNA-DNA杂交、基因组DNA G+C含量及系统发育分析将这两株分离菌分为两个不同的基因种，它们在脂肪酸谱、碳源利用模式、抗生素敏感性及生化特性方面也有明显差异。这两株分离菌在1.5%-20% NaCl存在的情况下生长，最适生长温度为28℃，最适pH值为7.5。菌株YIM D82(T)和YIM D812(T)的基因组DNA G+C含量分别为61.5 mol%和62.3 mol%。基于16S rRNA基因序列的系统发育分析表明，菌株YIM D82(T)和YIM D812(T)属于红螺菌科，与它们亲缘关系最近的索多玛红弧菌（Rhodovibrio sodomensis）和盐沼红弧菌（Rhodovibrio salinarum）的相似性分别为90.5%-90.6%和90.1%-90.2%。这两株分离菌与已确认属的不同表型和基因型特征表明，这两个菌株应被分类为代表一个新属和两个新物种，为此提出了新属名盐沼弯杆菌属（Fodinicurvata），新种名盐沼弯杆菌盐矿亚种（Fodinicurvata sediminis gen. nov., sp. nov.）（模式菌株YIM D82(T)=DSM 21159(T)=KCTC 22351(T)）和盐沼弯杆菌凤岗亚种（Fodinicurvata fenggangensis sp. nov.）（模式菌株YIM D812(T)=CCTCC AA 208037(T)=DSM 21160(T)）。
