Cai Man, Tang Shu-Kun, Chen Yi-Guang, Li Yan, Zhang Yu-Qin, Li Wen-Jun
The Key Laboratory for Microbial Resources of the Ministry of Education, Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan 650091, PR China.
Int J Syst Evol Microbiol. 2009 Oct;59(Pt 10):2471-5. doi: 10.1099/ijs.0.007682-0. Epub 2009 Jul 21.
Two novel halophilic, aerobic, catalase-positive but oxidase-negative, Gram-positive actinomycetes, designated YIM 91353(T) and YIM 91394(T), were isolated from a salt lake in the north-west of China. Phylogenetic analyses based on 16S rRNA gene sequences indicated that the novel isolates should be assigned to the genus Streptomonospora. The phenotypic and chemotaxonomic characteristics of the isolates also matched those described for members of the genus Streptomonospora. The predominant menaquinones were MK-10(H(8)), MK-10(H(6)) and MK-9(H(8)), and meso-diaminopimelic acid was the diagnostic amino acid in the cell walls. The phospholipids of the isolates consisted of diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannosides. The major fatty acids of strain YIM 91353(T) were anteiso-C(17 : 0) and C(18 : 0), and of strain YIM 91394(T) were anteiso-C(17 : 0) and iso-C(16 : 0). The DNA G+C contents were 71.2 and 72.5 mol%, respectively. The combination of phylogenetic analysis, DNA-DNA hybridization data, phenotypic characteristics and chemotaxonomic differences supported the view that strains YIM 91353(T) and YIM 91394(T) each represent a novel species of the genus Streptomonospora, for which the names Streptomonospora amylolytica sp. nov. and Streptomonospora flavalba sp. nov. are proposed, with type strains YIM 91353(T) (=DSM 45171(T)=CCTCC AA 208048(T)) and YIM 91394(T) (=DSM 45155(T)=CCTCC AA 208047(T)), respectively.
从中国西北部的一个盐湖中分离出两株新型嗜盐、需氧、过氧化氢酶阳性但氧化酶阴性的革兰氏阳性放线菌，分别命名为YIM 91353(T)和YIM 91394(T)。基于16S rRNA基因序列的系统发育分析表明，这些新分离株应归属于链单孢菌属。分离株的表型和化学分类学特征也与链单孢菌属成员的描述相符。主要的甲基萘醌为MK-10(H(8))、MK-10(H(6))和MK-9(H(8))，中-二氨基庚二酸是细胞壁中的诊断性氨基酸。分离株的磷脂由双磷脂酰甘油、磷脂酰胆碱、磷脂酰甘油、磷脂酰肌醇和磷脂酰肌醇甘露糖苷组成。菌株YIM 91353(T)的主要脂肪酸为anteiso-C(17 : 0)和C(18 : 0)，菌株YIM 91394(T)的主要脂肪酸为anteiso-C(17 : 0)和iso-C(16 : 0)。DNA G+C含量分别为71.2和72.5 mol%。系统发育分析、DNA-DNA杂交数据、表型特征和化学分类学差异的综合结果支持以下观点：菌株YIM 91353(T)和YIM 91394(T)各自代表链单孢菌属的一个新物种，为此提出了淀粉分解链单孢菌新种（Streptomonospora amylolytica sp. nov.）和黄白链单孢菌新种（Streptomonospora flavalba sp. nov.），其模式菌株分别为YIM 91353(T)（=DSM 45171(T)=CCTCC AA 208048(T)）和YIM 91394(T)（=DSM 45155(T)=CCTCC AA 208047(T)）。
