Department of Microbiology, Chung-Ang University College of Medicine, Seoul 06974, Republic of Korea.
Faculty of Arts and Science, University of Toronto, Toronto, Ontario M5S 1A1, Canada.
Int J Syst Evol Microbiol. 2024 Jul;74(7). doi: 10.1099/ijsem.0.006458.
A Gram-negative, non-motile, and creamy-white coloured bacterium, designated CAU 1616, was isolated from sea sand collected at Ayajin Beach, Goseong-gun, Republic of Korea. The bacterium was found to grow optimally at 37 °C, pH 8.0-8.5, and with 1-5 % (w/v) NaCl. Phylogenetic analyses based on the 16S rRNA gene sequences placed strain CAU 1616 within the order . The highest 16S rRNA gene sequence similarity was to YIM D812 (94.1 %), YIM D82 (93.7 %), BA45AL (93.6 %) and HHTR 118 (92.3 %). Comparing strain CAU 1616 with closely related species ( YIM D812 and YIM D82), the average nucleotide identity based on blast+ values were 69.7-69.8 %, the average amino acid identity values were 61.3-61.4 %, and the digital DNA-DNA hybridization values were 18.4-18.5 %. The assembled draft genome of strain CAU 1616 had 29 contigs with an N50 value of 385.8 kbp, a total length of 3 490 371 bp, and a DNA G+C content of 65.1 mol%. The predominant cellular fatty acids were C 2-OH, C cyclo 8, and summed feature 8 (C 6 and/or C 7). The major respiratory quinone was Q-10. Based on phenotypic, phylogenetic, and chemotaxonomic evidence, strain CAU 1616 represents a novel genus in the family , for which the name gen. nov., sp. nov. is proposed. The type strain is CAU 1616 (=KCTC 82428=MCCC 1K06089).
从韩国高城郡牙津海滩采集的海沙中分离到一株革兰氏阴性、非运动性、奶油白色的细菌，命名为 CAU 1616。该菌在 37°C、pH8.0-8.5 和 1-5%（w/v）NaCl 下生长最佳。基于 16S rRNA 基因序列的系统发育分析，菌株 CAU 1616 属于. 与最接近的种 YIM D812（94.1%）、 YIM D82（93.7%）、BA45AL（93.6%）和 HHTR 118（92.3%）相比，菌株 CAU 1616 的 16S rRNA 基因序列相似度最高。与密切相关的种（ YIM D812 和 YIM D82）相比，基于 blast+值的平均核苷酸同一性为 69.7-69.8%，平均氨基酸同一性值为 61.3-61.4%，数字 DNA-DNA 杂交值为 18.4-18.5%。CAU 1616 菌株的组装基因组草图有 29 个 contigs，N50 值为 385.8 kbp，总长度为 3490371 bp，GC 含量为 65.1 mol%。主要细胞脂肪酸为 C 2-OH、C 环 8 和总和特征 8（C 6 和/或 C 7）。主要呼吸醌为 Q-10。基于表型、系统发育和化学分类学证据，菌株 CAU 1616 代表了. 科的一个新属，建议使用新属名 gen. nov.，sp. nov.。模式菌株为 CAU 1616（=KCTC 82428=MCCC 1K06089）。
