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2
The bI4 group I intron binds directly to both its protein splicing partners, a tRNA synthetase and maturase, to facilitate RNA splicing activity.BI4 组 I 内含子直接与其两种蛋白质剪接伙伴（一种 tRNA 合成酶和成熟酶）结合，以促进 RNA 剪接活性。
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An inserted region of leucyl-tRNA synthetase plays a critical role in group I intron splicing.亮氨酰-tRNA合成酶的一个插入区域在I类内含子剪接中起关键作用。
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Synthesis and function of the mitochondrial intron--encoded bI4 RNA maturase from Saccharomyces cerevisiae. Effects of upstream frame-shift mutations.酿酒酵母线粒体内含子编码的bI4 RNA成熟酶的合成与功能。上游移码突变的影响。
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Connections between RNA splicing and DNA intron mobility in yeast mitochondria: RNA maturase and DNA endonuclease switching experiments.酵母线粒体中RNA剪接与DNA内含子移动性之间的联系：RNA成熟酶和DNA内切酶转换实验。
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In vitro mutagenesis of the mitochondrial leucyl tRNA synthetase of Saccharomyces cerevisiae shows that the suppressor activity of the mutant proteins is related to the splicing function of the wild-type protein.酿酒酵母线粒体亮氨酰tRNA合成酶的体外诱变表明，突变蛋白的抑制活性与野生型蛋白的剪接功能有关。
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The yeast mitochondrial leucyl-tRNA synthetase is a splicing factor for the excision of several group I introns.
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The DIVa maturase binding site in the yeast group II intron aI2 is essential for intron homing but not for in vivo splicing.酵母II组内含子aI2中的DIVa成熟酶结合位点对于内含子归巢至关重要，但对于体内剪接并非如此。
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The bI4 RNA mitochondrial maturase of Saccharomyces cerevisiae can stimulate intra-chromosomal recombination in Escherichia coli.
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Ccm1p is a 15S rRNA primary transcript processing factor as elucidated by a novel in vivo system in Saccharomyces cerevisiae.Ccm1p 是一种 15S rRNA 初级转录本加工因子，这是通过酿酒酵母中的一种新型体内系统阐明的。
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The Ribosome as a Missing Link in Prebiotic Evolution III: Over-Representation of tRNA- and rRNA-Like Sequences and Plieofunctionality of Ribosome-Related Molecules Argues for the Evolution of Primitive Genomes from Ribosomal RNA Modules.核糖体作为前生物进化的缺失环节 III：tRNA 和 rRNA 样序列的过度表达以及核糖体相关分子的多功能性表明，原始基因组是从核糖体 RNA 模块进化而来的。
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tRNA synthetase: tRNA aminoacylation and beyond.tRNA合成酶：tRNA氨基酰化及其他功能
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Two independent activities define Ccm1p as a moonlighting protein in Saccharomyces cerevisiae.两种独立的活性将 Ccm1p 定义为酿酒酵母中的一种兼职蛋白。
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Yeast mitochondrial leucyl-tRNA synthetase CP1 domain has functionally diverged to accommodate RNA splicing at expense of hydrolytic editing.酵母线粒体亮氨酰-tRNA 合成酶 CP1 结构域的功能已经分化，以适应 RNA 剪接，牺牲了水解编辑功能。
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本文引用的文献

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Aminoacyl tRNA synthetases and their connections to disease.氨酰-tRNA合成酶及其与疾病的关联。
Proc Natl Acad Sci U S A. 2008 Aug 12;105(32):11043-9. doi: 10.1073/pnas.0802862105. Epub 2008 Aug 5.
2
Structure of a tyrosyl-tRNA synthetase splicing factor bound to a group I intron RNA.与I组内含子RNA结合的酪氨酰-tRNA合成酶剪接因子的结构。
Nature. 2008 Jan 3;451(7174):94-7. doi: 10.1038/nature06413.
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Jekyll & Hyde: evolution of a superfamily.
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A viable amino acid editing activity in the leucyl-tRNA synthetase CP1-splicing domain is not required in the yeast mitochondria.酵母线粒体中不需要亮氨酰 - tRNA合成酶CP1剪接结构域中具有活性的氨基酸编辑活性。
J Biol Chem. 2006 Nov 3;281(44):33217-25. doi: 10.1074/jbc.M607406200. Epub 2006 Sep 6.
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Functional divergence of a unique C-terminal domain of leucyl-tRNA synthetase to accommodate its splicing and aminoacylation roles.亮氨酰-tRNA合成酶独特C末端结构域的功能分化，以适应其剪接和氨酰化作用。
J Biol Chem. 2006 Aug 11;281(32):23075-82. doi: 10.1074/jbc.M601606200. Epub 2006 Jun 14.
6
A tyrosyl-tRNA synthetase adapted to function in group I intron splicing by acquiring a new RNA binding surface.一种通过获得新的RNA结合表面而适应于在I组内含子剪接中发挥功能的酪氨酰-tRNA合成酶。
Mol Cell. 2005 Feb 4;17(3):417-28. doi: 10.1016/j.molcel.2004.12.026.
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The splicing of yeast mitochondrial group I and group II introns requires a DEAD-box protein with RNA chaperone function.酵母线粒体I类和II类内含子的剪接需要一种具有RNA伴侣功能的DEAD盒蛋白。
Proc Natl Acad Sci U S A. 2005 Jan 4;102(1):163-8. doi: 10.1073/pnas.0407896101. Epub 2004 Dec 23.
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Structural requirement for Mg2+ binding in the group I intron core.I组内含子核心中Mg2+结合的结构要求。
J Mol Biol. 2003 May 30;329(2):229-38. doi: 10.1016/s0022-2836(03)00430-3.
9
An inserted region of leucyl-tRNA synthetase plays a critical role in group I intron splicing.亮氨酰-tRNA合成酶的一个插入区域在I类内含子剪接中起关键作用。
EMBO J. 2002 Dec 16;21(24):6874-81. doi: 10.1093/emboj/cdf671.
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Structure-function relationships of the RNA-dependent RNA polymerase from poliovirus (3Dpol). A surface of the primary oligomerization domain functions in capsid precursor processing and VPg uridylylation.脊髓灰质炎病毒RNA依赖的RNA聚合酶（3Dpol）的结构-功能关系。初级寡聚化结构域的一个表面在衣壳前体加工和VPg尿苷酸化中发挥作用。
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亮氨酰 - tRNA合成酶依赖性和非依赖性激活I组内含子

Leucyl-tRNA synthetase-dependent and -independent activation of a group I intron.

作者信息

Boniecki Michal T, Rho Seung Bae, Tukalo Mikhail, Hsu Jennifer L, Romero Eliana P, Martinis Susan A

机构信息

Department of Biochemistry, University of Illinois, Urbana, Illinois 61801, USA.

出版信息

J Biol Chem. 2009 Sep 25;284(39):26243-50. doi: 10.1074/jbc.M109.031179. Epub 2009 Jul 21.

DOI:10.1074/jbc.M109.031179
PMID:19622748
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2785312/
Abstract

Leucyl-tRNA synthetase (LeuRS) is an essential RNA splicing factor for yeast mitochondrial introns. Intracellular experiments have suggested that it works in collaboration with a maturase that is encoded within the bI4 intron. RNA deletion mutants of the large bI4 intron were constructed to identify a competently folded intron for biochemical analysis. The minimized bI4 intron was active in RNA splicing and contrasts with previous proposals that the canonical core of the bI4 intron is deficient for catalysis. The activity of the minimized bI4 intron was enhanced in vitro by the presence of the bI4 maturase or LeuRS.

摘要

亮氨酰 - tRNA合成酶（LeuRS）是酵母线粒体内含子的一种必需的RNA剪接因子。细胞内实验表明，它与bI4内含子中编码的成熟酶协同发挥作用。构建了大的bI4内含子的RNA缺失突变体，以鉴定出一个结构完整适合进行生化分析的内含子。最小化的bI4内含子在RNA剪接中具有活性，这与之前认为bI4内含子的典型核心缺乏催化能力的观点形成对比。在体外，bI4成熟酶或LeuRS的存在增强了最小化的bI4内含子的活性。