Howorka Stefan, Siwy Zuzanna
Department of Chemistry, Institute of Structural Molecular Biology, University College London, London, UK WC1H 0AJ.
Chem Soc Rev. 2009 Aug;38(8):2360-84. doi: 10.1039/b813796j. Epub 2009 Jun 15.
In nanopore analytics, individual molecules pass through a single nanopore giving rise to detectable temporary blockades in ionic pore current. Reflecting its simplicity, nanopore analytics has gained popularity and can be conducted with natural protein as well as man-made polymeric and inorganic pores. The spectrum of detectable analytes ranges from nucleic acids, peptides, proteins, and biomolecular complexes to organic polymers and small molecules. Apart from being an analytical tool, nanopores have developed into a general platform technology to investigate the biophysics, physicochemistry, and chemistry of individual molecules (critical review, 310 references).
在纳米孔分析中，单个分子穿过单个纳米孔，导致离子孔电流中出现可检测的瞬时阻断。纳米孔分析因其简单性而受到欢迎，可使用天然蛋白质以及人造聚合物和无机孔来进行。可检测分析物的范围从核酸、肽、蛋白质和生物分子复合物到有机聚合物和小分子。除了作为一种分析工具外，纳米孔已发展成为一种通用的平台技术，用于研究单个分子的生物物理学、物理化学和化学（批判性综述，310篇参考文献）。
