[使用高效液相色谱法和二极管阵列检测器测定片剂中的乙酰水杨酸和水杨酸]
[Determination of acetylsalicylic and salicylic acid in tablets using high-performance liquid chromatography and a diode array detector].
作者信息
Nikolić M, Petrović D
机构信息
Vojnomedicinska akademija, Institut za farmaciju, Zavod za preventivnu medicinu.
出版信息
Vojnosanit Pregl. 1991 Jul-Aug;48(4):325-30.
A fast and sensitive method of high-performance liquid chromatography (HPLC) for determination of acetylsalicylic acid (ASA) and products of salicylic acid (SA) hydrolysis in tablets has been elaborated. Extraction was done with the mixture of methanol: formic acid (98:2 v/v) and separation of the components was achieved by using the colon of Ultropac Lichrosorb RP-18, 10 um, 4 cm x 250 mm and diode array detector. The mobile phase of methanol:water: :mol/1 H3PO4 (60:35:5) v/v, pP = 2.6 and safe flow of 1 ml/min were used. Follow up of chromatographic separation of ASA, SA and internal standard beta-naphtole (ITS) was performed at the wave length of 290 microns. The threshold of ASA was 0.5 mg/ml and of SA 0.0025 mg/ml.
已制定了一种快速灵敏的高效液相色谱法（HPLC），用于测定片剂中的乙酰水杨酸（ASA）和水杨酸（SA）水解产物。用甲醇：甲酸（98:2 v/v）混合物进行萃取，并使用10μm、4cm×250mm的Ultropac Lichrosorb RP - 18柱和二极管阵列检测器实现各组分的分离。使用甲醇：水：0.01mol/1 H3PO4（60:35:5）v/v的流动相，pH = 2.6，流速为1ml/min。在290微米波长下对ASA、SA和内标β-萘酚（ITS）进行色谱分离跟踪。ASA的检测限为0.5mg/ml，SA的检测限为0.0025mg/ml。
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