Wang C, Vickers T J, Mann C K
Athena Neurosciences, San Francisco, CA 94080, USA.
J Pharm Biomed Anal. 1997 Sep;16(1):87-94. doi: 10.1016/s0731-7085(97)00005-8.
A comparison was made between Raman and high-performance liquid chromatography (HPLC) analysis of aspirin tablets. The basis was an assay of aspirin content and the determination of salicylic acid produced by decomposition. Raman observations were performed directly on both intact and powdered tablet material. The limit of detection of HPLC with an ultraviolet detector is lower than that of the Raman measurement, but both are adequate for this application. The reproducibility of the Raman measurement is somewhat better than that of the HPLC measurement. Both methods were used in a degradation study in which samples were stored in a humid atmosphere for a maximum period of 8 weeks. Aside from somewhat higher salicylic acid responses from the HPLC method, which were attributed to hydrolysis during chromatography, results from the two methods were comparable. Direct Raman measurements are faster and do not require the use of solvents.
对阿司匹林片剂进行了拉曼光谱分析与高效液相色谱（HPLC）分析的比较。比较的基础是阿司匹林含量测定以及对分解产生的水杨酸的测定。拉曼光谱观察直接在完整片剂材料和粉末状片剂材料上进行。配备紫外检测器的HPLC的检测限低于拉曼测量的检测限，但两者都适用于此应用。拉曼测量的重现性比HPLC测量的重现性稍好。两种方法都用于一项降解研究，在该研究中，样品在潮湿气氛中储存最长8周。除了HPLC方法产生的水杨酸响应稍高（这归因于色谱过程中的水解）外，两种方法的结果具有可比性。直接拉曼测量更快，且无需使用溶剂。
