Yang Dong-Xia, Han Yan-Chun, Liu Lu-Ying, Yu Ning, Wang Xia, Shi Yan
Department of Pathology, Binzhou Medical College, Yantai, Shandong, 264003, People's Republic of China.
Ai Zheng. 2009 Jul;28(7):762-7. doi: 10.5732/cjc.008.10748.
The roles of vascular endothelial growth factor (VEGF) in tumor angiogenesis is related with Ets family. Elf-1, a member of Ets family, has seldom been studied. This study aimed to investigate the expression of Elf-1 and VEGF in non-small cell lung cancer (NSCLC), and explore their correlations to clinicopathologic features of NSCLC.
Tissue microarray containing 69 specimens of NSCLC and six specimens of normal lung tissues was constructed. The expression of Elf-1 and VEGF was detected by PowerVision-9000 immunohistochemistry.
Elf-1 and VEGF were not detected in all normal tissues; the positive rates of Elf-1 and VEGF were 72.46% and 63.77% in NSCLC, respectively. The expression levels of both Elf-1 and VEGF were significantly related with tumor differentiation, lymphatic metastasis, clinical stage, and postoperative survival time (all P < 0.01). Overexpression of them was related with poor prognosis: the survival rates were significantly lower in positive patients than in negative patients (both P < 0.01). Elf-1 expression was positively correlated to VEGF expression (r = 0.702, P < 0.01).
The expression of Elf-1 and VEGF in NSCLC is related to differentiation, lymphatic metastasis, clinical stage and prognosis. Detecting their expression in combination can help to predict the malignant behavior of NSCLC.
血管内皮生长因子（VEGF）在肿瘤血管生成中的作用与Ets家族有关。Ets家族成员之一Elf-1的研究较少。本研究旨在探讨Elf-1和VEGF在非小细胞肺癌（NSCLC）中的表达情况，并探讨它们与NSCLC临床病理特征的相关性。
构建包含69例NSCLC标本和6例正常肺组织标本的组织芯片。采用PowerVision-9000免疫组织化学法检测Elf-1和VEGF的表达。
所有正常组织均未检测到Elf-1和VEGF；NSCLC中Elf-1和VEGF的阳性率分别为72.46%和63.77%。Elf-1和VEGF的表达水平均与肿瘤分化、淋巴转移、临床分期及术后生存时间显著相关（均P < 0.01）。它们的过表达与预后不良有关：阳性患者的生存率显著低于阴性患者（均P < 0.01）。Elf-1表达与VEGF表达呈正相关（r = 0.702，P < 0.01）。
NSCLC中Elf-1和VEGF的表达与分化、淋巴转移、临床分期及预后有关。联合检测它们的表达有助于预测NSCLC的恶性行为。
