Ben-Dov Eitan, Ben Yosef Dafna Zeevi, Pavlov Valentina, Kushmaro Ariel
Department of Biotechnology Engineering, and National Institute for Biotechnology, Ben-Gurion University of the Negev, PO Box 653, Be'er-Sheva 84105, Israel.
Int J Syst Evol Microbiol. 2009 Oct;59(Pt 10):2458-63. doi: 10.1099/ijs.0.007468-0. Epub 2009 Jul 21.
A bacterial strain, designated Coryn-1(T), was isolated from mucus of the coral Fungia granulosa (northern Red Sea, Gulf of Eilat, Israel) by growth and enrichment of micro-organisms in agar spheres and subsequent plating. The bacterium was found to be a Gram-positive, non-motile, halotolerant, heterotrophic coccobacillus. Comparative 16S rRNA gene sequence analyses showed that strain Coryn-1(T) belonged to the genus Corynebacterium, exhibiting the highest levels of similarity (94 %) with the 16S rRNA gene sequence of Corynebacterium halotolerans YIM 70093(T). The novel strain grew well at 0.5-4.0 % salinity, at pH 7.2-9.0 and at 30-37 degrees C. The major cellular fatty acids were oleic acid (C(18 : 1)omega9c; 58 %), palmitic acid (C(16 : 0); 30 %) and tuberculostearic acid (10-methyl-C(18 : 0); 12 %). The DNA G+C content was 66.6 mol%. On the basis of its phenotypic properties and phylogenetic distinctiveness, strain Coryn-1(T) represents a novel species, for which the name Corynebacterium maris sp. nov. is proposed. The type strain is Coryn-1(T) (=DSM 45190(T)=LMG 24561(T)).
从珊瑚颗粒状石芝（红海北部，以色列埃拉特湾）的黏液中，通过在琼脂球中培养和富集微生物并随后进行平板接种，分离出了一株细菌，命名为Coryn-1(T)。该细菌为革兰氏阳性、不运动、耐盐、异养的球杆菌。16S rRNA基因序列比较分析表明，Coryn-1(T)菌株属于棒状杆菌属，与耐盐棒状杆菌YIM 70093(T)的16S rRNA基因序列相似度最高（94%）。该新菌株在盐度0.5-4.0%、pH值7.2-9.0和温度30-37℃条件下生长良好。主要细胞脂肪酸为油酸（C(18 : 1)ω9c；58%）、棕榈酸（C(16 : 0)；30%）和结核硬脂酸（10-甲基-C(18 : 0)；12%）。DNA G+C含量为66.6 mol%。基于其表型特征和系统发育独特性，Coryn-1(T)菌株代表一个新物种，为此提出新名称海棒状杆菌（Corynebacterium maris sp. nov.）。模式菌株为Coryn-1(T)（=DSM 45190(T)=LMG 24561(T)）。
