Elguezabal Natalia, Maza José L, Moragues María D, Pontón José
Department of Inmunología, Microbiología y Parasitología, Universidad del País Vasco, Bilbao, Spain.
Eur J Oral Sci. 2009 Aug;117(4):474-8. doi: 10.1111/j.1600-0722.2009.00642.x.
The aim of this study was to assess the role of whole saliva, four saliva-derived preparations, and six monoclonal antibodies (mAbs), directed against components of the cell wall of Candida albicans, on the adhesion of C. albicans and Candida dubliniensis to human epithelial cells (HEC). C. albicans serotype A NCPF 3153 and C. albicans serotype B ATCC 90028 showed higher adhesion to HEC than C. dubliniensis NCPF 3949. Pooled whole saliva was more efficient than salivary secretory immunoglobulin A, partially purified by chromatography, at inhibiting the adhesion of C. albicans serotype A NCPF 3153 to HEC. Monoclonal antibodies C7, 14-8, and 26G7 were the most potent inhibitors of adhesion. Our results show that mAbs can mimic the inhibition of adhesion of C. albicans to HEC that is mediated by human saliva.
本研究的目的是评估全唾液、四种唾液衍生制剂以及六种针对白色念珠菌细胞壁成分的单克隆抗体（mAb）对白色念珠菌和都柏林念珠菌黏附于人上皮细胞（HEC）的作用。白色念珠菌A型NCPF 3153和白色念珠菌B型ATCC 90028对HEC的黏附性高于都柏林念珠菌NCPF 3949。在抑制白色念珠菌A型NCPF 3153对HEC的黏附方面，混合全唾液比经色谱法部分纯化的唾液分泌型免疫球蛋白A更有效。单克隆抗体C7、14 - 8和26G7是最有效的黏附抑制剂。我们的结果表明，单克隆抗体可以模拟人唾液介导的对白色念珠菌黏附于HEC的抑制作用。
