San Millán Rosario, Elguezabal Natalia, Regúlez Pilar, Moragues Marı A Dolores, Quindós Guillermo, Pontón José
Departamento de Inmunologı́a, Microbiologı́a y Parasitologı́a, Facultad de Medicina y Odontologı́a1, and Departamento de Enfermerı́a I2, Universidad del Paı́s Vasco, Apartado 699, E-48080 Bilbao, Vizcaya, Spain.
Microbiology (Reading). 2000 Sep;146 ( Pt 9):2105-2112. doi: 10.1099/00221287-146-9-2105.
Attachment of Candida albicans to plastic materials of dental prostheses or to salivary macromolecules adsorbed on their surface is believed to be a critical event in the development of denture stomatitis. In an earlier study, it was shown that adhesion of C. albicans to polystyrene, a model system to study the adhesion of C. albicans to plastic materials, can be partially inhibited with an mAb directed against cell wall polysaccharides of C. albicans. In the present study, the role of whole saliva in the adhesion of C. albicans to polystyrene has been investigated, and three mAbs directed against epitopes of cell wall mannoproteins have been used to mimic the inhibitory effect observed with salivary secretory IgA (sIgA) on the adhesion of C. albicans to polystyrene. In the absence of whole saliva, adherence of C. albicans 3153 increased with germination. However, the presence of whole saliva enhanced the adhesion to polystyrene of C. albicans 3153 yeast cells but decreased the adhesion of germinated cells. The enhancement of adhesion of yeast cells to polystyrene mediated by saliva was confirmed with an agerminative mutant of C. albicans 3153. The inhibition of the adhesion of C. albicans 3153 germ tubes to polystyrene was due to the salivary sIgA since sIgA-depleted saliva enhanced the adhesion of C. albicans 3153 to polystyrene. The inhibitory effect mediated by sIgA was not related to the inhibition of germination but to the blockage of adhesins expressed on the cell wall surface of the germ tubes. The three mAbs studied reduced the adhesion of C. albicans 3153 to polystyrene at levels equivalent to those for purified sIgA. The highest reduction in the adhesion was obtained with the IgA mAb N3B. The best results were obtained when the three mAbs were combined. The results suggest that whole saliva plays a different role in the adhesion of C. albicans to polystyrene depending on the morphological phase of C. albicans. These results may give new insights into the conflicting role of saliva in the adhesion of C. albicans to plastic materials of dental prostheses.
白色念珠菌附着于假牙的塑料材料或其表面吸附的唾液大分子被认为是义齿性口炎发生过程中的一个关键事件。在早期研究中，已表明白色念珠菌对聚苯乙烯（一种用于研究白色念珠菌对塑料材料黏附的模型系统）的黏附可被一种针对白色念珠菌细胞壁多糖的单克隆抗体部分抑制。在本研究中，已对全唾液在白色念珠菌对聚苯乙烯黏附中的作用进行了研究，并且使用了三种针对细胞壁甘露糖蛋白表位的单克隆抗体来模拟唾液分泌型IgA（sIgA）对白色念珠菌黏附于聚苯乙烯的抑制作用。在没有全唾液的情况下，白色念珠菌3153的黏附随着发芽而增加。然而，全唾液的存在增强了白色念珠菌3153酵母细胞对聚苯乙烯的黏附，但降低了发芽细胞的黏附。白色念珠菌3153的无芽突变体证实了唾液介导的酵母细胞对聚苯乙烯黏附的增强。白色念珠菌3153芽管对聚苯乙烯黏附的抑制是由于唾液中的sIgA，因为去除sIgA的唾液增强了白色念珠菌3153对聚苯乙烯的黏附。sIgA介导的抑制作用与发芽抑制无关，而是与芽管细胞壁表面表达的黏附素的阻断有关。所研究的三种单克隆抗体使白色念珠菌3153对聚苯乙烯的黏附降低到与纯化的sIgA相当的水平。IgA单克隆抗体N3B使黏附降低的程度最高。三种单克隆抗体联合使用时效果最佳。结果表明，全唾液在白色念珠菌对聚苯乙烯的黏附中发挥的作用因白色念珠菌的形态阶段而异。这些结果可能为唾液在白色念珠菌黏附于假牙塑料材料中的矛盾作用提供新的见解。
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