Bouizgarne B, Lanoot B, Loqman S, Spröer C, Klenk H-P, Swings J, Ouhdouch Y
Faculté des Sciences Semlalia, Université Cadi Ayyad, Marrakech, Morocco.
Int J Syst Evol Microbiol. 2009 Nov;59(Pt 11):2857-63. doi: 10.1099/ijs.0.011387-0. Epub 2009 Jul 23.
The novel actinomycete strain Ap1(T) was isolated from rhizosphere soil of the argan tree (Argania spinosa L.) in the south of Morocco. Strain Ap1(T) has been reported as a novel producer of the pentaene polyene macrolide isochainin, which strongly inhibits the growth of pathogenic yeasts and phytopathogenic fungi. Strain Ap1(T) shows a greyish-white aerial mycelium with chains of smooth-surfaced spores of the Spiralis type and a cell wall containing ll-diaminopimelic acid. Based on chemotaxonomy and morphological features, strain Ap1(T) was identified as a member of the genus Streptomyces. 16S rRNA gene sequence similarities based on almost-complete 16S rRNA gene sequences showed that strain Ap1(T) is closely associated with members of the Streptomyces violaceoruber species group (S. violaceoruber, S. coelescens, S. violaceorubidus, 'S. caesius', 'S. lividans', S. violaceolatus and S. humiferus) and others (Streptomyces aurantiogriseus, S. lienomycini, S. chattanoogensis, S. rubrogriseus and S. tendae). However, protein profiling, DNA-DNA hybridization and BOX-PCR fingerprinting proved a relationship above the species level. In addition, the phenotype also allowed for the differentiation of strain Ap1(T) from its closest neighbours. As a result of this polyphasic approach, we conclude that strain Ap1(T) represents a novel species of the genus Streptomyces, for which the name Streptomyces marokkonensis sp. nov. is proposed. The type strain is Ap1(T) (=R-22003(T) =LMG 23016(T) =DSM 41918(T)).
新型放线菌菌株Ap1(T)是从摩洛哥南部阿甘树（Argania spinosa L.）的根际土壤中分离得到的。菌株Ap1(T)已被报道为五烯多烯大环内酯类异链菌素的新型产生菌，该物质能强烈抑制致病性酵母和植物病原真菌的生长。菌株Ap1(T)呈现灰白色气生菌丝体，具螺旋形表面光滑的孢子链，细胞壁含ll-二氨基庚二酸。基于化学分类学和形态学特征，菌株Ap1(T)被鉴定为链霉菌属的成员。基于几乎完整的16S rRNA基因序列的16S rRNA基因序列相似性表明，菌株Ap1(T)与紫红红链霉菌种组（紫红红链霉菌、天蓝色链霉菌、紫红红链霉菌、‘青色链霉菌’、‘变铅青链霉菌’、紫紫链霉菌和土栖链霉菌）的成员以及其他一些菌（橙灰链霉菌、里氏链霉菌、查塔努加链霉菌、红灰链霉菌和滕氏链霉菌）密切相关。然而，蛋白质谱分析、DNA-DNA杂交和BOX-PCR指纹图谱证明其亲缘关系高于种的水平。此外，该表型也使菌株Ap1(T)与其最相近的菌株得以区分。通过这种多相分类方法，我们得出结论，菌株Ap1(T)代表链霉菌属的一个新种，为此提出名为摩洛哥链霉菌新种（Streptomyces marokkonensis sp. nov.）。模式菌株为Ap1(T)（=R-22003(T)=LMG 23016(T)=DSM 41918(T)）。
